{"created":"2023-05-15T13:36:52.828079+00:00","id":460,"links":{},"metadata":{"_buckets":{"deposit":"d2103de3-9dd6-4fe3-80ca-f01bad70b710"},"_deposit":{"created_by":11,"id":"460","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"460"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000460","sets":["2:582:67","87:608:35","87:668:52"]},"author_link":["1260","2138","1974","3284","1957","1962"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-12","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"89","bibliographicPageStart":"85","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"北日本病害虫研究会報"},{"bibliographic_title":"Annual report of the Society of Plant Protection of North Japan","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"ジャガイモ黒あし病菌の人工汚染土を対象として，増菌PCR 法による検出を試みた．ペクチンを炭素源とする液体培地，Liquid Enrichment Medium（LEMAG366）中で25 ℃，5 日間の増菌培養後，増菌液からDNA を抽出してPCR 法に供試することにより，国内で発生している黒あし病菌3 菌種の検出が可能であった．培養容器としては取り扱いが容易なチャック付きラミネート袋が利用可能であり，増菌培養時の流動パラフィン重層の有無は検出結果に影響しなかった．本増菌PCR 法の検出限界は，Pectobacterium atrosepticum で乾土1 g あたり4×10２cfu，P. carotovorum で2×10１cfu，Dickeya sp.で3×10１cfu 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