{"created":"2023-05-15T13:36:37.083689+00:00","id":40,"links":{},"metadata":{"_buckets":{"deposit":"d24edda2-95e5-4931-b6b2-0d506be3ea41"},"_deposit":{"created_by":11,"id":"40","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"40"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000040","sets":["87:591:41","87:591:655:22:28"]},"author_link":["237","240","239","236","238"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03-24","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"34","bibliographicPageStart":"23","bibliographic_titles":[{"bibliographic_title":"農研機構研究報告　野菜花き研究部門"},{"bibliographic_title":"Bulletin of the NARO, Vegetable and Floriculture Science","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"1）年末から年明けにかけて収穫可能なハクサイ中晩性F1品種‘寒次郎’に複数の病原型グループの根こぶ病菌に対する抵抗性を付与するため，‘寒次郎’の両親(RKG42とTu3）に，‘はくさい中間母本農9号’（PL9）が有するCrr1とCrr2の2つの根こぶ病抵抗性遺伝子を導入しCMS_CR_RKG83とCR_Tu83およびそのF1の‘CR 寒次郎 ’を育成した．両親の育成には，6回の連続戻し交雑とDNAマーカーによる選抜を行った．\n2）選抜に用いたDNAマーカーは，Crr1が，RKG42とTu3ともにCrr1a抵抗性遺伝子末端に位置するB359であり，Crr2はRKG42が523A1R，Tu3がBRMS-096であった．いずれのマーカーの増幅断片はアガロースゲル電気泳動によりPL9と反復親（RKG42とTu3）間で明確に識別可能であった．\n3）病土挿入法による根こぶ病抵抗性検定の結果，CMS_CR_RKG83とCR_Tu83およびそのF1 の‘CR 寒次郎 ’は，4つの根こぶ病菌病原型グループの中で，グループ3に属する菌系には罹病したが，グループ1，2，4に抵抗性を示した．\n4）2カ所の根こぶ病菌汚染圃場に栽培した結果，いずれの圃場でも対照となる品種は罹病株が観察されたが，‘CR 寒次郎’は根こぶの着生が観察されず，圃場レベルでも安定した抵抗性を示した．\n5）‘CR 寒次郎’の根こぶ病抵抗性以外の特性は，元品種の‘寒次郎’のそれとよく似ており外観形質から判断することは困難であった．すなわち，‘CR 寒次郎’は12月～1月どりに適しており，約3㎏以上の球重と鮮やかな黄芯のハクサイであり，‘ 寒次郎’の後継品種として期待できる．","subitem_description_type":"Abstract"},{"subitem_description":"Two clubroot resistance genes，Crr1 and Crr2，derived from ‘Hakusai Chukanbohon Nou 9 Go’（PL9）were introduced into both parents（RKG42 and Tu3）of the mid-late maturing Chinese cabbage cultivar ‘Kanjiro’， which lacked clubroot resistance，using marker-assisted selection．F1 hybrids between each parent and PL9 were backcrossed with RKG42 or Tu3，and the progenies with two resistance genes were selected using B359， which is an InDel marker located on the in Crr1 and Crr2 linkage markers，523A1R for RKG42 and BRMS-096 for Tu3．The DNA marker selection and backcrossing were repeated six times and progenies homozygous for the two introduced resistance genes were obtained by self-pollination．These resistance genes were also introduced into cytoplasmic male sterile（CMS）RKG42 using the BC6F2 selection line，CR_RKG83，as a pollen parent．\nSubsequently，new parent lines，CMS_CR_RKG83 and CR_Tu83，harboring the two clubroot resistance genes and the F1 hybrid ‘CR Kanjiro’ were developed．The new parental lines and ‘CR Kanjiro’ showed resistance to three pathotypes，which were grouped based on differences in responses of the two CR cultivars，‘CR Ryutoku’\nand ‘SCR Hiroki’．The new F1 hybrid ‘CR Kanjiro’ showed high clubroot resistance in pot and field tests．\nPhenotypic characters of ‘CR Kanjiro’ were identical to those of ‘Kanjiro’ with medium-late maturation．","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00000030","subitem_identifier_reg_type":"JaLC"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2432-7859","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"松元, 哲"},{"creatorName":"マツモト, サトル","creatorNameLang":"ja-Kana"},{"creatorName":"MATSUMOTO, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"236","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"00355629","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=00355629"},{"nameIdentifier":"mats88","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/mats88"}]},{"creatorNames":[{"creatorName":"畠山, 勝徳"},{"creatorName":"ハタケヤマ, カツノリ","creatorNameLang":"ja-Kana"},{"creatorName":"HATAKEYAMA, Katsunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"237","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60355625","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60355625"}]},{"creatorNames":[{"creatorName":"高下, 新二"},{"creatorName":"タカシタ, シンジ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKASHITA, Shinji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"238","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000019036924","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000019036924"}]},{"creatorNames":[{"creatorName":"宮崎, 俊夫"},{"creatorName":"ミヤザキ, トシオ","creatorNameLang":"ja-Kana"},{"creatorName":"MIYAZAKI, Toshio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"239","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"近藤, 友宏"},{"creatorName":"コンドウ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"KONDO, Tomohiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"240","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-08-18"}],"displaytype":"detail","filename":"nivfs_report01-3p.pdf","filesize":[{"value":"12.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nivfs_report01-3p.pdf ","url":"https://repository.naro.go.jp/record/40/files/nivfs_report01-3p.pdf"},"version_id":"89070761-21ca-4079-adc3-ccb2d3a70641"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"2 つの根こぶ病抵抗性遺伝子（Crr1，Crr2）を有する ハクサイ中晩生F1 品種‘CR 寒次郎’ の育成","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"2 つの根こぶ病抵抗性遺伝子（Crr1，Crr2）を有する ハクサイ中晩生F1 品種‘CR 寒次郎’ の育成"},{"subitem_title":"Development of a Medium-Late Maturing Chinese Cabbage（Brassica rapa L．）F1 Cultivar ‘CR Kanjiro’，Harboring Two Clubroot Resistance Genes，Crr1 and Crr2","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["28","41"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-08-18"},"publish_date":"2017-08-18","publish_status":"0","recid":"40","relation_version_is_last":true,"title":["2 つの根こぶ病抵抗性遺伝子（Crr1，Crr2）を有する ハクサイ中晩生F1 品種‘CR 寒次郎’ の育成"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T16:09:55.810952+00:00"}