@article{oai:repository.naro.go.jp:00003814,
 author = {辻井, 直 and FUJIWARA-TSUJII, Nao and 安居, 拓恵 and YASUI, Hiroe and 永山, 敦士 and NAGAYAMA, Atsushi and 新垣, 則雄 and ARAKAKI, Norio},
 journal = {農研機構研究報告, Journal of the NARO Research and Development},
 month = {Jul},
 note = {The white grub beetle, Dasylepida ishigakiensis Niijima et Kinoshita (Coleoptera: Scarabaeidae), is an indigenous pest of sugarcane in the Miyako Islands, Okinawa, Japan. The mouthparts of the adult beetles are known to degenerate and they are assumed to have limited nutritional resources. On the basis of this, we studied changes in the quantity of stored lipids in the adult lifetime. For observing changes of stored lipid quantity, we adopt a commercial kit to conduct methylation of induced fatty acids in the whole insect body and purify them for analysis. We individually extracted and methylated whole lipids in the bodies of male beetles, both in laboratory-reared and feral individuals, and analyzed the methylated fatty acids by gas chromatography for quantification. In laboratory-reared males, we found no difference between newly eclosed beetles and those just after reproductive diapause, only the males who intensively flew for mating had lower levels of fatty acids. Feral males who have flown vigorously for mating possessed significant lower levels of fatty acid. From these results, we are able to know that there is certain relationship between male’s flight capability and stored lipids quantity in non-migratory insects.},
 pages = {11--16},
 title = {Quantitative analysis of total stored lipids in laboratory-reared and feral males of the white grub beetle, Dasylepida ishigakiensis},
 volume = {4},
 year = {2020},
 yomi = {ツジイ, ナオ and ヤスイ, ヒロエ and ナガヤマ, アツシ and アラカキ, ノリオ}
}