{"created":"2023-05-15T13:36:49.118730+00:00","id":374,"links":{},"metadata":{"_buckets":{"deposit":"db62da3f-9664-46dd-a09a-2bd17d737616"},"_deposit":{"created_by":11,"id":"374","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"374"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000374","sets":["87:627:628:17:99"]},"author_link":["366","1141","367","1599","916","325"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"58","bibliographicPageStart":"53","bibliographic_titles":[{"bibliographic_title":"農研機構研究報告 食品研究部門"},{"bibliographic_title":"Bulletin of the NARO,Food Research","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Protein NP24 found in tomato (Solanum lycopersicum L.) and PR5-like protein in sweet pepper (Capsicum annuum L.) are homologous proteins of osmotin. Osmotin is known to show adiponectin-like activity, and therefore, protein NP24 and PR5-like protein are expected to show similar activity. In this study, we quantified these proteins contained in the fruits of tomato and sweet pepper cultivars using protein absolute quantification (AQUA) technology employing liquid chromatography/tandem mass spectrometry (LC/MS/MS) and stable isotope-labeled internal standard peptide,GQTWVINAPR[13C6,15N4] (AQUA peptide). The range and average of protein NP24 content in 12 tomato cultivars were 0.43+-0.040 (‘Reika’) to 0.83+-0.40 (' Momotaro Fight' ) and 0.66+-0.14 nmol/g fresh weight, respectively. The PR5-like  protein content in 5 sweet pepper cultivars ranged from 0.079+-0.034 ('Ferrari’ harvested in September) to 0.54+- 0.14('Helsinki' harvested in December) nmol/g fresh weight. This study could contribute to research on the application of these proteins as adiponectin agonists and the use of tomato and sweet pepper as functional foods.\n","subitem_description_type":"Abstract"}]},"item_10002_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"トマト（Solanum lycopersicum L ）のタンパク質 NP24 およびパプリカ(Capsicum annuum L）の PR5 様タンパク質は，オスモチンの相同タンパク質である．オスモチンは，アディポネクチン様活性を示すことが知られており，それ故に，タンパク質 NP24 および PR5 様タンパク質は，同様の活性を示すことが期待されている． この研究において，我々は，液体クロマトグラフィー／タンデムマススペクトロメトリー（LC/MS/MS）と安定同位体標識内部標準ペプチド GQTWVINAPR[13C6,15N4] を用いたタンパク質絶対定量法（AQUA）により，トマトおよびパプリカの果実中のタンパク質 NP24 および PR5 様タンパク質を定量した．トマト 12 品種を測定したころ 0.43+-0.040 （‘麗夏’）～ 0.83+-0.40（‘桃太郎ファイト’）nmol/g 新鮮重で，平均値は0.66+-0.14nmol/g 新鮮重であった．パプリカ品種を測定したところ  0.079+-0.034( 9 月収穫の‘フェラーリ’）～  0.54+- 0.14(12月収穫の‘ヘルシンキ’)nmol/g  新鮮重であった．この研究は，これらタンパク質のアディポネクチンアゴニストおよびトマトやパプリカの機能性食品としての利用に関する研究に貢献するかもしれない．","subitem_description_type":"Other"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00000351","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構"},{"subitem_publisher":"National Agriculture and Food Research Organization (NARO)"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00000351","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2432-7891","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"一法師, 克成"},{"creatorName":"イッポウシ, カツナリ","creatorNameLang":"ja-Kana"},{"creatorName":"IPPOSHI, Katsunari","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"斎藤, 新"},{"creatorName":"サイトウ, アツシ","creatorNameLang":"ja-Kana"},{"creatorName":"SAITO, Atsushi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"ISHIKAWA-TAKANO, Yuko","creatorNameLang":"en"},{"creatorName":"石川（高野）, 祐子","creatorNameLang":"ja"},{"creatorName":"イシカワ（タカノ）, ユウコ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKANO-ISHIKAWA, Yuko","creatorNameLang":"en"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"大池, 秀明"},{"creatorName":"オオイケ, ヒデアキ","creatorNameLang":"ja-Kana"},{"creatorName":"OIKE, Hideaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"小堀, 真珠子"},{"creatorName":"コボリ, マスコ","creatorNameLang":"ja-Kana"},{"creatorName":"KOBORI, Masuko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"山本（前田）, 万里"},{"creatorName":"ヤマモト（マエダ）, マリ","creatorNameLang":"ja-Kana"},{"creatorName":"MAEDA-YAMAMOTO, Mari","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-16"}],"displaytype":"detail","filename":"nfri_kenkyuhoukoku02_09.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_kenkyuhoukoku02_09.pdf","url":"https://repository.naro.go.jp/record/374/files/nfri_kenkyuhoukoku02_09.pdf"},"version_id":"ad972ab2-0acd-47d3-ad9e-5fd00929cf35"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"protein NP24","subitem_subject_scheme":"Other"},{"subitem_subject":"PR5-like protein","subitem_subject_scheme":"Other"},{"subitem_subject":"osmotin-like protein","subitem_subject_scheme":"Other"},{"subitem_subject":"tomato","subitem_subject_scheme":"Other"},{"subitem_subject":"sweet pepper","subitem_subject_scheme":"Other"},{"subitem_subject":"protein NP24","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PR5-like protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"osmotin-like protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"tomato","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"sweet pepper","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Quantification of protein NP24 in tomato and PR5-like protein in  sweet pepper","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Quantification of protein NP24 in tomato and PR5-like protein in  sweet pepper"},{"subitem_title":"Quantification of protein NP24 in tomato and PR5-like protein in  sweet pepper","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["99"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-05-16"},"publish_date":"2018-05-16","publish_status":"0","recid":"374","relation_version_is_last":true,"title":["Quantification of protein NP24 in tomato and PR5-like protein in  sweet pepper"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-07-20T06:24:00.731932+00:00"}