{"created":"2023-05-15T13:39:11.444614+00:00","id":3335,"links":{},"metadata":{"_buckets":{"deposit":"e405e2d2-f188-4d15-b64c-39fbf4bd7476"},"_deposit":{"created_by":11,"id":"3335","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"3335"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003335","sets":["1706970004180:1:420:437","87:647:47"]},"author_link":["625","670","1310","6937","6939","6938"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-12-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"62","bibliographicPageStart":"57","bibliographicVolumeNumber":"2","bibliographic_titles":[{"bibliographic_title":"農研機構研究報告"},{"bibliographic_title":"Journal of the NARO Research and Development","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Suzumaru R was developed by introducing three resistant genes against soybean cyst nematode, namely, rhg1, rhg2, and Rhg4 into Suzumaru by backcrossing six times and marker-assisted selection. In the present study, we performed whole genome resequencing of the soybean varieties Suzumaru and Suzumaru R and compared the sequence for isogeneity confi rmation of the genetic backgrounds of the varieties. At the genome level, Suzumaru and Suzumaru R were found to have the same genetic ackground, using a sliding window analysis, except for three fragments, each of which contained rhg1, rhg2, or Rhg4, and a 3.6-Mb fragment at the end of chromosome 5. In the breeding process, the end of chromosome 5 was intentionally selected for soybean dwarf virus resistance (Rsdv1) from the donor parent in the BC6F1 to BC6F4 generation. However, plants having Rsdv1 showed unfavorable traits, and therefore, plants that did not have Rsdv1 were selected eventually. This small fragment on chromosome 5 might be a remnant region. Thus, whole-genome re-sequencing can be a powerful tool for in-depth analyses of the difference between two varieties. Applying this technology to backcross breeding would enable “precision breeding.” \n\nセンチュウ抵抗性ダイズ品種「スズマル R」と反復親の「スズマル」の遺伝的背景の同質性確認のため，全ゲノムリシーケンスを行い，塩基配列を比較した．その結果，「スズマル R」に残存する「スズマル」以外のゲノム断片は，シストセンチュウ抵抗性遺伝子を含む 3 箇所と，第 5 染色体末端の 3.6 Mb の小さな断片のみであることがわかった．第5染色体の断片は，当初導入が試みられていたダイズわい化病抵抗性遺伝子（Rsdv1）領域に近接していた．この断片は，選抜の最終段階で不良形質発現のため Rsdv1 を「スズマル」型に戻した際に近隣領域に残った導入断片であると考えられた．本研究により，全ゲノムシーケンスによる配列比較により「スズマル」と「スズマル R」の遺伝的背景の高い同質性を明らかにした．全ゲノムリシーケンス解析は，遺伝的背景を詳細に比較可能にする強力なツールであり，DNAマーカーでは検出が困難な小さな導入断片まで特定することで，より精密なピンポイント育種に貢献できると考えられる．","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00003208","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構"},{"subitem_publisher":"National Agriculture and Food Research Organization (NARO) "}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.34503/naroj.2019.2_57","subitem_relation_type_select":"DOI"}}]},"item_10002_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"本文はJ-Stageよりご利用いただけます。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This article full-text PDF is available at J-Stage."}]}]},"item_10002_relation_17":{"attribute_name":"全文リンク","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"https://doi.org/10.34503/naroj.2019.2_57"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.34503/naroj.2019.2_57","subitem_relation_type_select":"DOI"}}]},"item_10002_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"2022.2 J-Stageへ移管しました"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"We changed the journal platform to J-Stage.　2022.2 -"}]}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2434-9909","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"小木曽-田中 , 映理","creatorNameLang":"ja"},{"creatorName":"オギソ-タナカ, エリ","creatorNameLang":"ja-Kana"},{"creatorName":"OGISO-TANAKA, Eri","creatorNameLang":"en"},{"creatorName":"小木曽, 映里","creatorNameLang":"ja"},{"creatorName":"オギソ, エリ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{},{},{}],"givenNames":[{},{},{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"竹内, 徹"},{"creatorName":"タケウチ, トオル","creatorNameLang":"ja-Kana"},{"creatorName":"TAKEUCHI, Toru","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"山下, 陽子"},{"creatorName":"ヤマシタ, ヨウコ","creatorNameLang":"ja-Kana"},{"creatorName":"YAMASHITA, Yoko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"黒崎, 英樹"},{"creatorName":"クロサキ, ヒデキ","creatorNameLang":"ja-Kana"},{"creatorName":"KUROSAKI, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"TAGUCHI-SHIOBARA, Fumio","creatorNameLang":"en"},{"creatorName":"TAGUCHI, Fumio","creatorNameLang":"en"},{"creatorName":"田口, 文緒","creatorNameLang":"ja"},{"creatorName":"タグチ, フミオ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"羽鹿, 牧太"},{"creatorName":"ハジカ, マキタ","creatorNameLang":"ja-Kana"},{"creatorName":"HAJIKA, Makita","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2022-04-15"}],"displaytype":"detail","filename":"10.24514 00003208_DOI_moved.pdf","filesize":[{"value":"298.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"10.24514 00003208_DOI_moved","url":"https://repository.naro.go.jp/record/3335/files/10.24514 00003208_DOI_moved.pdf"},"version_id":"e4a1fd9f-f0ee-45e2-9665-b3d592464270"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ダイズ","subitem_subject_scheme":"Other"},{"subitem_subject":"全ゲノムリシーケンス","subitem_subject_scheme":"Other"},{"subitem_subject":"戻し交配","subitem_subject_scheme":"Other"},{"subitem_subject":"マーカー選抜","subitem_subject_scheme":"Other"},{"subitem_subject":"Soybean","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Whole genome re-sequence","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Backcross","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Marker-assisted selection","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ゲノムから見た「スズマル」と「スズマル R」の同質性","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ゲノムから見た「スズマル」と「スズマル R」の同質性"},{"subitem_title":"Identity of Suzumaru and Suzumaru R Revealed by Whole Genome Sequencing","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["47","437"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-25"},"publish_date":"2019-12-25","publish_status":"0","recid":"3335","relation_version_is_last":true,"title":["ゲノムから見た「スズマル」と「スズマル R」の同質性"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-07-20T06:31:57.134104+00:00"}