{"created":"2023-05-15T13:39:00.781889+00:00","id":3094,"links":{},"metadata":{"_buckets":{"deposit":"89ddcef8-0980-4b99-be94-9b6fca50dbb0"},"_deposit":{"created_by":12,"id":"3094","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"3094"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003094","sets":["87:627:628:388:403"]},"author_link":["6621","6795","2044"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"93","bibliographicPageStart":"87","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A multidrug efflux pump, AcrAB-TolC, contributes to multiple antibiotic resistance (Mar) phenotype in Escherichia coli. In this study, we investigated multidrug tolerance mediated by genomic segmental duplication of acrAB locus in E. coli. In a strain K-12 W3110, 3.8% (8 of 208) of ampicillin-resistant colonies carried the multiplicated copy of acrA gene, thereby exhibiting multidrug-resistance phenotype. In contrast, in a strain O157:H7 MY-29, we could not isolate such mutant. All isolated Mar strains were found to carry the same 416 kb-genomic amplicon. Sequencing analysis showed that the amplified regions were bordered by insertion sequence (IS), IS5. These results suggest that E. coli gene amplification occurs largely between directly oriented homologous IS elements. Thus, our results suggest that the genomic amplification-mediated Mar phenotype depends on the genetic background.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002970","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002970","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"本山, 志織"},{"creatorName":"モトヤマ, シオリ","creatorNameLang":"ja-Kana"},{"creatorName":"MOTOYAMA, Shiori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6795","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000405869715","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000405869715"}]},{"creatorNames":[{"creatorName":"ワナラット, ワナシリ"},{"creatorName":"ワナラット, ワナシリ","creatorNameLang":"ja-Kana"},{"creatorName":"WANNARAT, Wannasiri","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6621","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"稲岡, 隆史"},{"creatorName":"イナオカ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"INAOKA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2044","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40391205","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40391205"},{"nameIdentifier":"read0134908","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0134908"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-09"}],"displaytype":"detail","filename":"nfri_report_No80p87-93p.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No80p87-93p.pdf","url":"https://repository.naro.go.jp/record/3094/files/nfri_report_No80p87-93p.pdf"},"version_id":"5832046e-622e-4c7e-8cb9-e030073cb37e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"genomic amplification","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"multidrug tolerance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Escherichia coli","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"大腸菌におけるゲノム重複を介した多剤耐性化","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大腸菌におけるゲノム重複を介した多剤耐性化"},{"subitem_title":"Multidrug tolerance mediated by genomic segmental amplification in Escherichia coli","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["403"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"3094","relation_version_is_last":true,"title":["大腸菌におけるゲノム重複を介した多剤耐性化"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:33:45.634575+00:00"}