@article{oai:repository.naro.go.jp:00003094,
 author = {本山, 志織 and MOTOYAMA, Shiori and ワナラット, ワナシリ and WANNARAT, Wannasiri and 稲岡, 隆史 and INAOKA, Takashi},
 journal = {食品総合研究所研究報告, Report of National Food Research Institute},
 month = {Mar},
 note = {A multidrug efflux pump, AcrAB-TolC, contributes to multiple antibiotic resistance (Mar) phenotype in Escherichia coli. In this study, we investigated multidrug tolerance mediated by genomic segmental duplication of acrAB locus in E. coli. In a strain K-12 W3110, 3.8% (8 of 208) of ampicillin-resistant colonies carried the multiplicated copy of acrA gene, thereby exhibiting multidrug-resistance phenotype. In contrast, in a strain O157:H7 MY-29, we could not isolate such mutant. All isolated Mar strains were found to carry the same 416 kb-genomic amplicon. Sequencing analysis showed that the amplified regions were bordered by insertion sequence (IS), IS5. These results suggest that E. coli gene amplification occurs largely between directly oriented homologous IS elements. Thus, our results suggest that the genomic amplification-mediated Mar phenotype depends on the genetic background.},
 pages = {87--93},
 title = {大腸菌におけるゲノム重複を介した多剤耐性化},
 volume = {80},
 year = {2016},
 yomi = {モトヤマ, シオリ and ワナラット, ワナシリ and イナオカ, タカシ}
}