{"created":"2023-05-15T13:39:00.607888+00:00","id":3090,"links":{},"metadata":{"_buckets":{"deposit":"fdb76caf-2b76-4897-b041-745a04e7d2f1"},"_deposit":{"created_by":12,"id":"3090","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"3090"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003090","sets":["87:627:628:388:403"]},"author_link":["6637","5110","6636","5109","5108","953","1037","1657","2887"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"68","bibliographicPageStart":"57","bibliographicVolumeNumber":"80","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The use of genetically modified (GM) stacked-event products combining two or more GM parental events, especially in maize, has been increasing. This situation has made real-time PCR-based quantitative methods prone to overestimation of the content of GM organisms (GMOs) relative to the actual weight proportion. To solve this problem, we previously proposed a group testing method enabling accurate weight-based evaluation of GMO content in maize grains. In the present study, this method was slightly modified and validated in-house in order to enhance its reliability and user friendliness. Additionally, we developed a testing method for identification of GM events in the lysate samples used for the group testing. First, we confirmed specificity of the modified method by means of authentic GM and non-GM materials. Evaluation of the limit of detection suggested that the modified method reliably detected a single GM kernel in a group sample composed of 20 maize kernels. Then, we conducted a blind test using simulated group samples. All of the testing results matched the presence/ absence of GM kernels in the simulated samples. Furthermore, identification of GM events in the group testing samples was successfully achieved by DNA purification from the residual lysates and subsequent real-time PCR array analyses.","subitem_description_type":"Abstract"},{"subitem_description":"複数の遺伝子組換え (GM) 系統の掛け合わせによるスタック品種の使用拡大に伴い, リアルタイムPCRによる定量検知法は, GMトウモロコシの混入率を実際よりも過大に評価する傾向にある. この問題を解決するため, トウモロコシ穀粒中の組換え体混入率を正確に評価することができるグループテスティング法をこれまでに提案している. 本研究では, 既報の分析法の利便性および信頼性を向上させるため, 分析法を改変し, 室内妥当性確認を行った. また, グループテスティング後にGM系統を特定する試験法を確立した. 最初に, トウモロコシの各種GM系統及び非組換え体の純粋な試料を用いて, 改変法の反応特異性を確認した. また, 検出限界の評価を行い, 改変法が20粒中に１粒含まれるGM穀粒を確実に検出できることを確認した. 次に, 擬似混入試料を用いたブラインド試験を実施した. 全ての擬似混入試料で, 試料中のGMの有無に対応した分析結果が得られた. また, グループテスティングで使用した細胞溶解液からDNAを精製し, それをリアルタイムPCRアレイ法で分析することで, グループテスティングの試料に含まれていたGM系統の正確な特定が可能であった. ","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002966","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002966","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"真野, 潤一"},{"creatorName":"マノ, ジュンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"MANO, Junichi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"高島, かおり"},{"creatorName":"タカシマ, カオリ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKASHIMA, Kaori","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"布藤 , 聡"},{"creatorName":"フトウ, サトシ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"峯岸 , 恭孝"},{"creatorName":"ミネギシ, ヤスタカ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"二宮, 健二"},{"creatorName":"ニノミヤ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"NINOMIYA, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"野口 , 秋雄"},{"creatorName":"ノグチ, アキオ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"近藤 , 一成"},{"creatorName":"コンドウ, カズナリ","creatorNameLang":"ja-Kana"},{"creatorName":"Kondo, Kazunari","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"高畠, 令王奈"},{"creatorName":"タカバタケ, レオナ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKABATAKE, Reona","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"橘田, 和美"},{"creatorName":"キッタ, カズミ","creatorNameLang":"ja-Kana"},{"creatorName":"KITTA, Kazumi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-09"}],"displaytype":"detail","filename":"nfri_report_No80p57-68p.pdf","filesize":[{"value":"991.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No80p57-68p.pdf","url":"https://repository.naro.go.jp/record/3090/files/nfri_report_No80p57-68p.pdf"},"version_id":"47c3f7fe-0f94-40e9-80f7-4e84861f9813"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"遺伝子組換え","subitem_subject_scheme":"Other"},{"subitem_subject":"グループテスティング","subitem_subject_scheme":"Other"},{"subitem_subject":"妥当性確認","subitem_subject_scheme":"Other"},{"subitem_subject":"GMO","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"group testing","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"validation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"遺伝子組換えトウモロコシの混入率評価を目的とするグループテスティング法の改良","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"遺伝子組換えトウモロコシの混入率評価を目的とするグループテスティング法の改良"},{"subitem_title":"Improvement of the group testing method to evaluate GM maize content","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["403"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"3090","relation_version_is_last":true,"title":["遺伝子組換えトウモロコシの混入率評価を目的とするグループテスティング法の改良"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:44:03.674954+00:00"}