{"created":"2023-05-15T13:38:59.858628+00:00","id":3072,"links":{},"metadata":{"_buckets":{"deposit":"0fa42ce5-dd8c-4d4b-a306-9e3f3901f680"},"_deposit":{"created_by":12,"id":"3072","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"3072"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003072","sets":["87:627:628:388:402"]},"author_link":["315","1704","6699"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"76","bibliographicPageStart":"67","bibliographicVolumeNumber":"79","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In order to produce ethanol from xylose, which was the second most abundant sugar in cellulosic biomass, the xylose reductase, xylitol dehydrogenase, and xylulokinase genes were overexpressed in three different Saccharomyces cerevisiae strains, ATCC 24860, InvSc1, and NBRC 0224. The recombinant strain derived from S. cerevisiae ATCC 24860 was expected to possess the high xylose-fermenting ability owing to its high ability of ethanol production from xylulose, which was an intermediate of the xylose metabolism. In contrast to the expectation, the recombinant strain of ATCC 24860 did not show higher xylose fermentation compared to the other recombinant strains. This result suggests that the conversion efficiency from xylose to xylulose is more critical for conferring the xylose-fermenting ability on S. cerevisiae by introducing the exogenous pathway consisting of xylose reductase and xylitol dehydrogenase, rather than the xylulose-fermenting ability of the host cells.","subitem_description_type":"Abstract"},{"subitem_description":"セルロース系バイオマスに多く含まれるキシロースを効率的にエタノールに変換するために, キシロースレダクターゼ, キシリトールデヒドロゲナーゼ, キシルロキナーゼ遺伝子を, 3つの異なる Saccharomyces cerevisiae 株において高発現させた. このうち, キシロースの代謝中間産物であるキシルロースの発酵能が高い ATCC 24860 株に由来する組換え株は, 高いキシロース発酵能を示すことが期待されたが, 実際には, 他の２つの株由来のものに対する優位性は見出されなかった. キシロースレダクターゼ及びキシリトールデヒドロゲナーゼの導入によって S. cerevisiae にキシロース発酵能を付与する際には, キシルロース以降の代謝よりも, キシロースからキシルロースへの代謝により留意する必要がある.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002948","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002948","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"榊原, 祥清"},{"creatorName":"サカキバラ, ヨシキヨ","creatorNameLang":"ja-Kana"},{"creatorName":"SAKAKIBARA, Yoshikiyo","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"王, 暁輝"},{"creatorName":"ワン, シャオフィ","creatorNameLang":"ja-Kana"},{"creatorName":"WANG, Xiaohui","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"徳安, 健"},{"creatorName":"トクヤス, ケン","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-08"}],"displaytype":"detail","filename":"nfri_report_No79p67-76p.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No79p67-76p.pdf","url":"https://repository.naro.go.jp/record/3072/files/nfri_report_No79p67-76p.pdf"},"version_id":"bede3d7e-8aab-4072-88ee-16a07b0c591e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"バイオエタノール","subitem_subject_scheme":"Other"},{"subitem_subject":"サッカロミセス・セレビジエ","subitem_subject_scheme":"Other"},{"subitem_subject":"キシロース発酵","subitem_subject_scheme":"Other"},{"subitem_subject":"キシルロース発酵","subitem_subject_scheme":"Other"},{"subitem_subject":"Bioethanol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Saccharomyces cerevisiae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Xylose fermentation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Xylulose fermentation","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"宿主株が異なるSaccharomyces cerevisiae 組換え体におけるキシロース発酵能の比較","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"宿主株が異なるSaccharomyces cerevisiae 組換え体におけるキシロース発酵能の比較"},{"subitem_title":"Comparative evaluation of ethanol production from xylose by recombinant Saccharomyces cerevisiae derived from different host strains","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["402"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"3072","relation_version_is_last":true,"title":["宿主株が異なるSaccharomyces cerevisiae 組換え体におけるキシロース発酵能の比較"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:43:03.062198+00:00"}