{"created":"2023-05-15T13:38:58.896021+00:00","id":3047,"links":{},"metadata":{"_buckets":{"deposit":"fea6cd51-c0db-464a-86fe-1f64e3b9c626"},"_deposit":{"created_by":12,"id":"3047","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"3047"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003047","sets":["87:627:628:388:401"]},"author_link":["6795","6757","2044"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"41","bibliographicPageStart":"37","bibliographicVolumeNumber":"78","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Gene amplification, reiteration of a chromosomal DNA segment, spontaneously occurs during replication in all organisms. In this study, we investigated B. subtilis gene amplification using a chloramphenicol-resistance gene (cat) as a selection marker. When B. subtilis strain TI74 carrying a single copy set of cat and β-galactosidase gene (lacZ) on the genome was plated onto the medium containing chloramphenicol at the higher dosages than its minimum inhibitory concentration, it was found that all high-level resistant strains carried the multi-copy of cat gene. Furthermore, the lacZ-amplifications were also observed in the cat-amplified strains, thereby resulting in the increase in β-galactosidase activity. These results indicate that the cat-amplified strains can be selected for increased chloramphenicol resistance efficiently. Thus, cat gene is thought to be useful as a selection marker for gene amplification in B. subtilis.","subitem_description_type":"Abstract"},{"subitem_description":"ゲノム重複を活用した微生物育種法の開発を目指し, その効果を枯草菌で検証した. Cm耐性遺伝子 (cat) をゲノム重複株の選抜用マーカーとして用いたところ, 単離した全ての株でゲノム重複が生じており, cat 遺伝子がゲノム重複株の選抜に極めて有効であることが示された. また, cat 遺伝子と共に重複させたβ-ガラクトシダーゼ遺伝子においても, 酵素活性を約５倍まで増加させることができた.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002926","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002926","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"稲岡, 隆史"},{"creatorName":"イナオカ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"INAOKA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2044","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40391205","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40391205"},{"nameIdentifier":"read0134908","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0134908"}]},{"creatorNames":[{"creatorName":"草房, 克江"},{"creatorName":"クサフサ, カツエ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSAFUSA, Katsue","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6757","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000405869714","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000405869714"}]},{"creatorNames":[{"creatorName":"本山, 志織"},{"creatorName":"モトヤマ, シオリ","creatorNameLang":"ja-Kana"},{"creatorName":"MOTOYAMA, Shiori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6795","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000405869715","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000405869715"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-08"}],"displaytype":"detail","filename":"nfri_report_No78p37-41p.pdf","filesize":[{"value":"441.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No78p37-41p.pdf","url":"https://repository.naro.go.jp/record/3047/files/nfri_report_No78p37-41p.pdf"},"version_id":"7f94baf4-9551-41e0-832d-a15810b9b25a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"クロラムフェニコール耐性遺伝子によるゲノム重複を活用した枯草菌育種法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"クロラムフェニコール耐性遺伝子によるゲノム重複を活用した枯草菌育種法"},{"subitem_title":"Investigation of Bacillus subtilis gene amplification by utilizing chloramphenicol-resistance gene","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["401"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"3047","relation_version_is_last":true,"title":["クロラムフェニコール耐性遺伝子によるゲノム重複を活用した枯草菌育種法"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:33:43.967816+00:00"}