{"created":"2023-05-15T13:38:57.356936+00:00","id":3011,"links":{},"metadata":{"_buckets":{"deposit":"cf28a84c-0a19-4cdd-b7c5-944f90d53a16"},"_deposit":{"created_by":12,"id":"3011","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"3011"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003011","sets":["87:627:628:388:399"]},"author_link":["6681","6671","6609","6656","6740","551"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"50","bibliographicPageStart":"45","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Epoxide hydrolases (EH) catalyze hydrolysis of epoxides to the corresponding vicinal diols. By site-directed mutagenesis, a total of 22 mutants were constructed, followed by analyzing their kinetics. Through these experiments, mutation of G32, S39, and D58, located at a highly conserved region of EHs known to date, led to a 2-fold higher specific activity than that of wild-type.","subitem_description_type":"Abstract"},{"subitem_description":"エポキシド水解酵素は, エポキシドを加水分解し, ジオールに変換する触媒作用を示す. ダイズエポキシド水解酵素の22種の部位特異的変異体の作出を試み,  6種の可溶性変異酵素が得られた. 得られた変異酵素の内, ゲノム上極めて高度に保存されている領域に変異を有する 3種の酵素については, 野生型酵素の 2倍近い酵素活性が得られた. 野生型酵素及び変異酵素のCDスペクトルを測定した結果, 顕著な差異は見られなかった. 変異による酵素活性の上昇はアミノ酸変異による微少な変化を反映しているものと予想された.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002891","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002891","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"荒平, 正緒美"},{"creatorName":"アラヒラ, マサオミ","creatorNameLang":"ja-Kana"},{"creatorName":"ARAHIRA, Masaomi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"551","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20353930","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20353930"},{"nameIdentifier":"masao_ara","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/masao_ara"}]},{"creatorNames":[{"creatorName":"SAILAS, Benjamin"},{"creatorName":"SAILAS, Benjamin","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6609","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000000030080","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000000030080"}]},{"creatorNames":[{"creatorName":"LEE, Sam-Pin"},{"creatorName":"LEE, Sam-Pin","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6671","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000257906198","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000257906198"}]},{"creatorNames":[{"creatorName":"NGHIEM, Ngoc Minh"},{"creatorName":"NGHIEM, Ngoc Minh","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"PHAN, Van Chi"},{"creatorName":"PHAN, Van Chi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6681","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000252815207","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000252815207"}]},{"creatorNames":[{"creatorName":"深澤, 親房"},{"creatorName":"フカザワ, チカフサ","creatorNameLang":"ja-Kana"},{"creatorName":"FUKAZAWA, Chikafusa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6740","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000003063582","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000003063582"},{"nameIdentifier":"read0004151","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0004151"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-07"}],"displaytype":"detail","filename":"nfri_report_No76p45-50p.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No76p45-50p.pdf","url":"https://repository.naro.go.jp/record/3011/files/nfri_report_No76p45-50p.pdf"},"version_id":"a5ab7bd2-5a59-46bf-9b53-a113c512ef34"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"epoxide hydrolase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mutant","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ダイズ種子エポキシド加水分解酵素変異体の作出及びその性質検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ダイズ種子エポキシド加水分解酵素変異体の作出及びその性質検討"},{"subitem_title":"Characterization of soybean epoxide hydrolase mutant","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["399"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"3011","relation_version_is_last":true,"title":["ダイズ種子エポキシド加水分解酵素変異体の作出及びその性質検討"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T16:03:06.604736+00:00"}