{"created":"2023-05-15T13:38:57.183948+00:00","id":3007,"links":{},"metadata":{"_buckets":{"deposit":"58439699-a2f0-4787-a205-1b43daf2bdd1"},"_deposit":{"created_by":12,"id":"3007","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"3007"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00003007","sets":["87:627:628:388:399"]},"author_link":["6718"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2012-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"28","bibliographicPageStart":"23","bibliographicVolumeNumber":"76","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An inulin fructotransferase (DFA III-producing) [EC 2.4.1.93] from Arthrobacter nicotinovorans K-9 was purified and characterized. The enzyme was purified 35.7-fold from the culture supernatant of the microorganism with a yield of 12.1%. The enzyme showed maximum activity at 60℃ and pH 6.0, and its activity was stable up to 70℃ after 30 min of heat treatment. Its molecular mass was estimated to be 40 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 70 kDa by gel filtration, and the enzyme was considered to be a dimer.","subitem_description_type":"Abstract"},{"subitem_description":"新たに分離したDFA III オリゴ糖合成酵素生産菌K-9株について分類学的検討を行った結果, 本菌はArthrobacter nicotinovorans と同定された. 本菌株のDFA III 合成酵素を粗酵素液である培養上清からDEAE-トヨパール, Butyl-トヨパール, SuperQ-トヨパールによるクロマトグラフィーにより, 電気泳動的に均一に精製した. 本酵素の反応至適pH は6.0, 反応至適温度は60℃であった. 本酵素の耐熱性について検討すると70℃まで安定という結果が得られた. 本酵素の分子量について検討するとSDS-電気泳動から40KDa, ゲル濾過から70KDa という値が得られた. このため本酵素はホモダイマーと推察される.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002887","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002887","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"原口, 和朋"},{"creatorName":"ハラグチ, カズトモ","creatorNameLang":"ja-Kana"},{"creatorName":"HARAGUCHI, Kazutomo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6718","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006426314","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006426314"},{"nameIdentifier":"90353961","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90353961"},{"nameIdentifier":"read0134913","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0134913"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-07"}],"displaytype":"detail","filename":"nfri_report_No76p23-28p.pdf","filesize":[{"value":"944.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No76p23-28p.pdf","url":"https://repository.naro.go.jp/record/3007/files/nfri_report_No76p23-28p.pdf"},"version_id":"96ff9d6c-dd02-47e2-87e9-0e728e98531e"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Arthrobacter, DFA III (difructose dianhydride III), inulin, enzyme, purification","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Arthrobacter nicotinovorans K-9 のInulin fructotransferase (DFA III-producing)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Arthrobacter nicotinovorans K-9 のInulin fructotransferase (DFA III-producing)"},{"subitem_title":"Inulin Fructotransferase (DFA III-producing) From Arthrobacter nicotinovorans K-9","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["399"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"3007","relation_version_is_last":true,"title":["Arthrobacter nicotinovorans K-9 のInulin fructotransferase (DFA III-producing)"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T23:04:22.542698+00:00"}