@article{oai:repository.naro.go.jp:00002974,
 author = {箭田, 浩士 and YADA, Hiroshi and 谷中, かおる and YANAKA, Kaoru and 亀山, 眞由美 and OHNISHI-KAMEYAMA, Mayumi},
 journal = {食品総合研究所研究報告, Report of National Food Research Institute},
 month = {Mar},
 note = {The method of HPLC sample preparation for 5-hydorxymethyl-2-furfural (HMF) quantification in honey was developed. This method carried out as follows, dilute honey with ethanol/water =70/30 (v/v), centrifuge the solution, and filtrate the centrifugal supernatant with 0.2μm membrane filter. HPLC samples prepared from the filtrate by dilution with water, which were indicated stable analytical values of HMF during one-night(16hrs). This method is useful for routine HMF quantification in honey, because no waste solution containing zinc acetate. We also described the HPLC condition, gradient elution with C18 column/ detection at 254 nm and 284 nm. This HPLC condition make easy to detect the peak which changed from HMF., The method of HPLC sample preparation for 5-hydorxymethyl-2-furfural (HMF) quantification in honey was developed. This method carried out as follows, dilute honey with ethanol/water =70/30 (v/v), centrifuge the solution, and filtrate the centrifugal supernatant with 0.2μm membrane filter. HPLC samples prepared from the filtrate by dilution with water, which were indicated stable analytical values of HMF during one-night(16hrs). This method is useful for routine HMF quantification in honey, because no waste solution containing zinc acetate. We also described the HPLC condition, gradient elution with C18 column/ detection at 254 nm and 284 nm. This HPLC condition make easy to detect the peak which changed from HMF.},
 pages = {7--13},
 title = {はちみつに含まれるHMF 定量分析法の開発},
 volume = {74},
 year = {2010},
 yomi = {ヤダ, ヒロシ and ヤナカ, カオル and カメヤマ, マユミ}
}