{"created":"2023-05-15T13:38:55.732940+00:00","id":2973,"links":{},"metadata":{"_buckets":{"deposit":"b32d66c2-99a6-4b0a-a761-508e29549981"},"_deposit":{"created_by":12,"id":"2973","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2973"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002973","sets":["87:627:628:388:397"]},"author_link":["1471","352","353"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"5","bibliographicPageStart":"1","bibliographicVolumeNumber":"74","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To elucidate the molecular mechanism underlying the toxicity of the Fusarium mycotoxin nivalenol, we investigated the involvement of ryanodine receptor 1 (RyR1), an intracellular calcium ion channel, in nivalenol-induced cytotoxicity in human promyelocytic leukemia HL60 cells by using an RyR1-specific inhibitor dantrolene. Cell proliferation and cytokine levels were investigated after 24-h treatment with the chemicals. Nivalenol markedly hindered cell proliferation, while dantrolene retarded moderately. If RyR1 is not concerned with the nivalenol signal-transduction pathway(s), dantrolene should magnify the inhibitory effect of nivalenol. However, dantrolene did not strengthen the effect of nivalenol, therefore, RyR1 might play a role in the nivalenol-associated retardation of cell proliferation. Nivalenol significantly elicited interleukin (IL)-8 secretion, while dantrolene did not. Dantrolene substantially attenuated the effect of nivalenol, indicating that RyR1 is important for nivalenol-induced IL-8 secretion. Nivalenol reduced monocyte chemotactic protein (MCP)-1 secretion, while dantrolene decreased moderately. Dantrolene clearly alleviated the effect of nivalenol, indicating that RyR1 mediates the nivalenol-induced inhibition of MCP-1 secretion. Taken together the above results, the involvement of RyR1 in nivalenol-induced toxicity in HL60 cells was deduced.","subitem_description_type":"Abstract"},{"subitem_description":"ニバレノール (NIV) の毒性発現機構を解明するために, 細胞内カルシウムチャンネルであるリアノジン受容体 (RyR1) のHL60細胞におけるNIV 誘導性の細胞毒性への関与を, RyR1特異的阻害剤のダントロレンを用いて検討した。それぞれのアッセイは薬剤の24時間処理後に行った。NIV は顕著に細胞増殖を阻害したが, ダントロレンによる阻害は穏やかであった。もしRyR1がNIV のシグナル伝達経路に関係ないのであれば, ダントロレンがNIV による阻害作用を増強することが期待される。ダントロレンはNIV の作用を増強しなかったことから, RyR1はNIV による細胞増殖阻害に何らかの役割を果たしているのかもしれない。NIV は顕著にインターロイキン (IL) -8の分泌を誘導したが, ダントロレンはIL-8分泌量を変化させなかった。ダントロレンはNIV の影響を十分に減じたので, RyR1がNIV 誘導性のIL-8分泌に重要であると考えられた。NIV とダントロレンは単球走化性タンパク質 (MCP) -の分泌を減少させたが, ダントロレンによる減少は穏やかだった。ダントロレンは明らかにNIV の作用を和らげたので, RyR1はNIV によるMCP-分泌阻害に関与していると考えられた。以上の結果から, NIV のHL60細胞に対する細胞毒性の発現にはRyR1が関与していると考えられた。","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002854","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002854","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"長嶋, 等"},{"creatorName":"ナガシマ, ヒトシ","creatorNameLang":"ja-Kana"},{"creatorName":"NAGASHIMA, Hitoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中川, 博之"},{"creatorName":"ナカガワ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAGAWA, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"久城, 真代"},{"creatorName":"クシロ, マサヨ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSHIRO, Masayo","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-07"}],"displaytype":"detail","filename":"nfri_report_No74p1-5p.pdf","filesize":[{"value":"105.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No74p1-5p.pdf","url":"https://repository.naro.go.jp/record/2973/files/nfri_report_No74p1-5p.pdf"},"version_id":"03828969-bc9d-4375-bb5c-f2df9c2d5c95"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"dantrolene","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"interleukin-8","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"monocyte chemotactic protein-1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"nivalenol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"ryanodine receptor","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"リアノジン受容体の阻害剤ダントロレンはHL60細胞におけるニバレノール誘導性の細胞毒性を軽減する","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リアノジン受容体の阻害剤ダントロレンはHL60細胞におけるニバレノール誘導性の細胞毒性を軽減する"},{"subitem_title":"Ryanodine receptor inhibitor dantrolene alleviates nivalenol-induced cytotoxicity in HL60 cells","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["397"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2973","relation_version_is_last":true,"title":["リアノジン受容体の阻害剤ダントロレンはHL60細胞におけるニバレノール誘導性の細胞毒性を軽減する"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:49:28.046012+00:00"}