@article{oai:repository.naro.go.jp:00002944,
 author = {中川, 博之 and NAKAGAWA, Hiroyuki and 亀山, 眞由美 and OHNISHI-KAMEYAMA, Mayumi and 佐合, 由紀 and SAGO, Yuki and 箭田, 浩士 and YADA, Hiroshi and 久城, 真代 and KUSHIRO, Masayo and 長嶋, 等 and NAGASHIMA, Hitoshi},
 journal = {食品総合研究所研究報告, Report of National Food Research Institute},
 month = {Mar},
 note = {Aflatoxins (AFs) are the most potent carcinogenic and toxic compounds among many kinds of substances in the nature, and benzo［a］pyrene (BaP) is known as a hardly degradable compound and also recognized as a carcinogen . Simultaneous detection of AFs (AFB_1, AFB_2 , AFG_1, and AFG_2) and BaP was performed by liquid ch romatography /tandem mass spectrometry (LC/MS /MS) equipped with an atomospheric pressure photo‒ionization (APPI) probe. Mixtures of AFs and BaP were applied to multi‒functional columns suggested for the purification on an official method for AFs determination, and both of them were well eluted through an Autoprep MF‒A1000 column. In the aim of practical usage for food analysis, AFs and BaP were added to olive oil at the concentration of 4.0μg/kg and 40μg/kg, respectively, and analyzed with LC/ MS/MS. When AFs and BaP were purified from olive oil with a sosid phase extraction column Autoprep MF‒A1000, the mean recovery of BaP was low (12.8 %) in contrast to that of AFs (102.1‒125.3%). The cause of low recovery of BaP was its low extraction efficiency from olive oil with the solvent of acetonitrile‒water (90: 10). If the purification process is improved to increase the extraction efficiency of BaP from olive oil , our method must be useful for the analysis of AFs and BaP contaminated in various foods., 大気圧光イオン化 (APPI) プローブを装着した高速液体クロマトグラフタンデム型質量分析装置 (LC/MS/MS) を使用して, アフラトキシン (AFs) 4 種 (AFB_1,AFB_2, AFG_1, AFG_2) とベンゾ［a］ピレン (BaP) の同時定量分析を行った. AFs とBaP の混合溶液についてAF 測定通知法で推奨されている3 種類の多機能固相抽出カラムを用いて前処理を行ったところ, AutoprepMF‒A1000 を用いた場合に両者を同時に精製することが可能であった. 実際の食品中におけるAFs とBaP の複合汚染を想定して, オリーブ油重量当たりAFs とBaP をそれぞれ4.0μg/kg, 40μg/kg の濃度で添加した試料の分析を行ったところ, Autoprep MF‒A1000を前処理に用いた場合, AFs の平均回収率は102.1 ～125.3%であったがBaPの平均回収率は12.8%であり, アセトニトリル‒水混合液 (90：10) ではオリーブ油中のBaP は十分に抽出できていないことが示唆された. 今回はAFs 測定通知法の前処理法を適用したが, 両者を効率良く抽出することができれば, 実サンプルでの同時分析は可能であるといえる.},
 pages = {45--52},
 title = {LC/MS/MS によるアフラトキシンとベンゾ［ａ］ピレンの同時分析},
 volume = {72},
 year = {2008},
 yomi = {ナカガワ, ヒロユキ and カメヤマ, マユミ and サゴウ, ユキ and ヤダ, ヒロシ and クシロ, マサヨ and ナガシマ, ヒトシ}
}