{"created":"2023-05-15T13:38:54.054013+00:00","id":2943,"links":{},"metadata":{"_buckets":{"deposit":"5873565d-a97a-42d0-974b-177b8b271033"},"_deposit":{"created_by":12,"id":"2943","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2943"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002943","sets":["87:627:628:388:395"]},"author_link":["2434","1471","352","353"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"44","bibliographicPageStart":"37","bibliographicVolumeNumber":"72","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Fumonisins are mycotoxins mainly produced by Fusarium verticillioides, which is a major pollutant to corn. On the other hand, rice adherent fungus Gibberella fujikuroi is taxonomically near to F.verticillioides, so that the potential risk of fumonisin contamination in rice is significant. Previously we developed an efficient extraction procedure (submergence extraction) and a sensitive detection method (liquid chromatography with tandem mass spectrometry (LC‒MS /MS)) for the determination of fumonisins in brown rice. In this study, we attempted to detect fumonisins in rice seed in the husk by an extension of the method for fumonisin analysis in brown rice, according to the request to detect fumonisins in rice before threshing. As a result, extraction with submergence was found to be effective in rice seed as well as in brown rice, but more effective way was to use a different solid phase extraction (SPE) cartridge for the purification of fumonisins from rice seed extract. With submergence extraction and purification with the newly selected SPE cartridge, fine recovery was achieved for clean rice seed samples in spike and recovery tests. About five hundred grams of rice seed sample was divided to seven subsamples, and ten grams from each subsample was subjected to analysis. The conventional determination method, liquid chromatography using fluorescence detection (HPLC‒FL) with precolumn derivatization, could not detect any fumonisins in any of the analytical samples, while fumonisins were detected in two of seven samples by use of LC‒MS /MS. This is the first case of the natural occurrence of fumonisins in domestic Japanese rice. The importance of sampling in non‒homogene‒ous samples like rice seed was also suggested.","subitem_description_type":"Abstract"},{"subitem_description":"フモニシンは主にトウモロコシ赤カビ病菌Fusarium verticillioides によって産生され, 広くトウモロコシを汚染するカビ毒であるが, コメに付着するイネ馬鹿苗病菌 Gibberella fujikuroi は, F.verticillioides の近縁であるため, コメでフモニシン汚染が懸念されていた. われわれは以前, 玄米中フモニシン定量のための効率的な抽出法 (浸漬抽出) ならびに高感度な液体クロマトグラフィー‒タンデム質量分析法 (LC‒MS/MS 法) を開発していた. 今回, 脱穀前段階でのフモニシン検出の要請があったため, 玄米中フモニシン分析法のマトリクスエクステンションを試みた. その結果, 抽出ステップでは, 玄米と同様に浸漬抽出が有効であった. 一方, 精製ステップでは, 玄米と異なる固相抽出カートリッジを用いるのが有効であり, ブランクの分析サンプルの添加回収試験において, 良好な回収率が得られた. 実際の種籾供試サンプル約500g を7個のサブサンプルに分けて分析を行った結果, 従来定量法であるプレカラム誘導体化機能付き液体クロマトグラフィー‒蛍光検出法 (HPLC‒FL 法) では, 各分析サンプル中のフモニシン量は検出限界未満であったが, LC‒MS/MS 法では, 7点中 2点が検出限界以上で測定可能であった. 今回の結果より, 国内産米で初めてフモニシンの検出に成功し, 種籾のように不均一な試料におけるサンプリングの重要性も示された.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002825","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002825","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"久城, 真代"},{"creatorName":"クシロ, マサヨ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSHIRO, Masayo","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"永田, 礼子"},{"creatorName":"ナガタ, レイコ","creatorNameLang":"ja-Kana"},{"creatorName":"NAGATA, Reiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"中川, 博之"},{"creatorName":"ナカガワ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAGAWA, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"長嶋, 等"},{"creatorName":"ナガシマ, ヒトシ","creatorNameLang":"ja-Kana"},{"creatorName":"NAGASHIMA, Hitoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-04"}],"displaytype":"detail","filename":"nfri_report_No72p37-44p.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No72p37-44p.pdf","url":"https://repository.naro.go.jp/record/2943/files/nfri_report_No72p37-44p.pdf"},"version_id":"2127a365-b0c7-473e-aa7c-ec535d3e0147"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Fumonisin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"rice","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gibberella fujikuroi","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"HPLC‒FL","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"LC‒MS /MS","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"種籾中フモニシン類の液体クロマトグラフィーによる検出","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"種籾中フモニシン類の液体クロマトグラフィーによる検出"},{"subitem_title":"Liquid chromatographic detection of fumonisins in rice seed","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["395"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2943","relation_version_is_last":true,"title":["種籾中フモニシン類の液体クロマトグラフィーによる検出"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:18:26.764289+00:00"}