{"created":"2023-05-15T13:38:53.988200+00:00","id":2942,"links":{},"metadata":{"_buckets":{"deposit":"bf14abea-52fe-4128-80f1-e9080f95168e"},"_deposit":{"created_by":12,"id":"2942","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2942"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002942","sets":["87:627:628:388:395"]},"author_link":["328","6768"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"35","bibliographicPageStart":"31","bibliographicVolumeNumber":"72","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Molecular assembly and the chain conformation of intact porcine stomach mucin in dilute solution were characterized by using low‒angle laser light scattering and small‒angle X‒ray scattering methods. The specific refractive index increment of the mucin was estimated to be 0.16 ml/g to determine the molecular weight by the low‒angle laser light scattering photometry combined with high performance gel chromatography . Weight‒averaged molecular weight of the mucin was 8.9 million and the molecular weight of the two main fractionated components was about 4 million and several hundred thousand. Fractal analysis of small‒angle X‒ray scattering data revealed that the intact mucin molecule is a chain with excluded volume (fractal dimension is 1.67). Moreover, the Kratky plots of the SAXS data showed that the chain conformation of the mucin molecules is an unfolded structure in solution.","subitem_description_type":"Abstract"},{"subitem_description":"食品分野における蛋白質の特性解析は, 蛋白質の素材としての有効利用に資することが期待される. 本研究では, ブタの胃粘液の主要成分のムチンの溶液構造特性の一端を解明するために, レーザー光散乱測定による分子量と分子量分布評価および放射光溶液X線散乱測定による分子鎖構造解析を行った. その結果, ブタ胃粘液ムチンの重量平均分子量は約890万であった. さらに, 分子量約400万と数十万の主要分子と比較的少量の分子量数百万以上の分子が構成要素であることが光散乱測定で明らかとなった. 一方, 放射光溶液X 線散乱測定から溶液中のブタ胃粘液ムチンは, ほどけた細長いフレキシブルな構造体であることがわかった. これら溶液散乱法による複合蛋白質の構造解析を通して, 広く食品関連生体高分子素材の溶液構造の解析に資する研究手法の提示も本研究の目的とした.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002824","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002824","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"渡邊, 康"},{"creatorName":"ワタナベ, ヤスシ","creatorNameLang":"ja-Kana"},{"creatorName":"WATANABE, Yasushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"328","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30353957","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30353957"},{"nameIdentifier":"001-001","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/001-001"}]},{"creatorNames":[{"creatorName":"猪子, 洋二"},{"creatorName":"イノコ, ヨウジ","creatorNameLang":"ja-Kana"},{"creatorName":"INOKO, Yoji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6768","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000000257438","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000000257438"},{"nameIdentifier":"read0013786","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0013786"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-04"}],"displaytype":"detail","filename":"nfri_report_No72p31-35p.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No72p31-35p.pdf","url":"https://repository.naro.go.jp/record/2942/files/nfri_report_No72p31-35p.pdf"},"version_id":"5df13489-1def-4688-8c98-83b5251e3172"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"粘液糖蛋白質の溶液物性評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"粘液糖蛋白質の溶液物性評価"},{"subitem_title":"Structural Characterization of a Mucous Glycoprotein in Solution","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["395"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2942","relation_version_is_last":true,"title":["粘液糖蛋白質の溶液物性評価"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T16:05:22.396416+00:00"}