{"created":"2023-05-15T13:38:53.006772+00:00","id":2927,"links":{},"metadata":{"_buckets":{"deposit":"e5de8559-8937-4a75-92f2-5f57bacd51f1"},"_deposit":{"created_by":12,"id":"2927","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2927"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002927","sets":["87:627:628:388:394"]},"author_link":["1632","6676"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"60","bibliographicPageStart":"57","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Erythrose reductase reduces D-erythrose into erythritol, a low calorie sweetner. Erythrose redutases have been identified in erythritol- producing fungi (Trichosporonoides megachiliensis), while yeast orthologues are still unknown. Gcy1p, known as a Saccharomyces cerevisiae aldose-reductase, possessed significant homology with the fungal erythrose reductases. The recombinant Gcy1p, expressed in Escherichia coli, showed similar substrate specificity towards D-erythrose and glycerol using NADPH as a reductant. Moreover, the Gcy1p reduced D-erythrose-4-phosphate as efficiently as D-erythrose. These facts suggest that S.cerevisiae has the potential to produce erythritol.","subitem_description_type":"Abstract"},{"subitem_description":"酵母Gcy1pはNADPH依存性のアルドースリダクターゼである. 大腸菌で発現させた組換え型Gcy1pは, トリコスポロノイデスメガチリエンスのエリスロースリダクターゼと同様にC-4及びC-3アルデヒドに対する基質特異性を示した. さらに, 酵母Gcy1pはエリスロース-4-リン酸も還元した. このことは, エリスリトールを産生しないとされてきた酵母においても, Gcy1pとホスファターゼ存在下では, エリスリトールが産生できる可能性を示している.","subitem_description_type":"Abstract"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002809","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大倉, 哲也"},{"creatorName":"オオクラ, テツヤ","creatorNameLang":"ja-Kana"},{"creatorName":"OOKURA, Tetsuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1632","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70353935","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70353935"},{"nameIdentifier":"fri","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/fri"}]},{"creatorNames":[{"creatorName":"春見, 隆文"},{"creatorName":"カスミ, タカフミ","creatorNameLang":"ja-Kana"},{"creatorName":"KASUMI, Takafumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6676","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000001350240","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000001350240"},{"nameIdentifier":"read0004157","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0004157"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-04"}],"displaytype":"detail","filename":"nfri_report_No71p57-60p.pdf","filesize":[{"value":"75.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No71p57-60p.pdf","url":"https://repository.naro.go.jp/record/2927/files/nfri_report_No71p57-60p.pdf"},"version_id":"99827eed-1519-4dc9-9977-22c8a2c5718a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"erythritol","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"pentose-phosphate shunt","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"erythrose reductase","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Gcy1p","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"酵母Gcy1pはエリスロースとエリスロース－4－リン酸を還元する","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"酵母Gcy1pはエリスロースとエリスロース－4－リン酸を還元する"},{"subitem_title":"Yeast Gcy1p Reduces Erythrose and Erythrose-4-phosphate","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["394"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2927","relation_version_is_last":true,"title":["酵母Gcy1pはエリスロースとエリスロース－4－リン酸を還元する"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:47:06.707712+00:00"}