{"created":"2023-05-15T13:38:50.978457+00:00","id":2893,"links":{},"metadata":{"_buckets":{"deposit":"438f4a22-e8c3-44b3-9bbb-44e596b65b10"},"_deposit":{"created_by":11,"id":"2893","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2893"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002893","sets":["2:581:62","87:551:680:50"]},"author_link":["2387"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"90","bibliographicPageStart":"84","bibliographicVolumeNumber":"205","bibliographic_titles":[{"bibliographic_title":"Journal of Structural Biology"},{"bibliographic_title":"Journal of Structural Biology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Isoprimeverose-producing enzymes (IPases) release isoprimeverose (α-d-xylopyranosyl-(1 → 6)-d-glucopyranose) from the non-reducing end of xyloglucan oligosaccharides. Aspergillus oryzae IPase (IpeA) is classified as a member of the glycoside hydrolase family 3 (GH3); however, it has unusual substrate specificity compared with other GH3 enzymes. Xylopyranosyl branching at the non-reducing ends of xyloglucan oligosaccharides is vital for IpeA activity. We solved the crystal structure of IpeA with isoprimeverose at 2.4 Å resolution, showing that the structure of IpeA formed a dimer and was composed of three domains: an N-terminal (β/α)8 TIM-barrel domain, α/β/α sandwich fold domain, and a C-terminal fibronectin-like domain. The catalytic TIM-barrel domain possessed a catalytic nucleophile (Asp300) and acid/base (Glu524) residues. Interestingly, we found that the cavity of the active site of IpeA was larger than that of other GH3 enzymes, and subsite −1′ played an important role in its activity. The glucopyranosyl and xylopyranosyl residues of isoprimeverose were located at subsites −1 and −1′, respectively. Gln58 and Tyr89 contributed to the interaction with the xylopyranosyl residue of isoprimeverose through hydrogen bonding and stacking effects, respectively. Our findings provide new insights into the substrate recognition of GH3 enzymes.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jsb.2018.11.005","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC30201901250001"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"author manuscript"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"このアーカイブは著者版です。出版社版ではありません。引用の際には出版社版をご利用ください。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This is not the published version. Please cite only the published version. "}]},{"subitem_relation_name":[{"subitem_relation_name_text":"Green open Access /This journal has an embargo period of 12 months."}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Journal of Structural Biology"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jsb.2018.11.005","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The Author(s) "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1047-8477","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"藤本, 瑞"},{"creatorName":"フジモト, ズイ","creatorNameLang":"ja-Kana"},{"creatorName":"FUJIMOTO, Zui","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-02-01"}],"displaytype":"detail","filename":"SC30201901250001_PrePrint-a.pdf","filesize":[{"value":"8.6 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC30201901250001_PrePrint","url":"https://repository.naro.go.jp/record/2893/files/SC30201901250001_PrePrint-a.pdf"},"version_id":"df40138e-da36-47f9-9b23-b5683110bcf1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Xyloglucan","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoprimeverose","subitem_subject_scheme":"Other"},{"subitem_subject":"Aspergillus oryzae","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycoside hydrolase family 3","subitem_subject_scheme":"Other"},{"subitem_subject":"Hemicellulose","subitem_subject_scheme":"Other"},{"subitem_subject":"Xyloglucan","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoprimeverose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Aspergillus oryzae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycoside hydrolase family 3","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hemicellulose","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme"},{"subitem_title":"Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["50","62"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-10-04"},"publish_date":"2019-10-04","publish_status":"0","recid":"2893","relation_version_is_last":true,"title":["Crystal structure and substrate recognition mechanism of Aspergillus oryzae isoprimeverose-producing enzyme"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-02-22T01:48:13.569659+00:00"}