{"created":"2023-05-15T13:38:46.530206+00:00","id":2802,"links":{},"metadata":{"_buckets":{"deposit":"42074986-bdc9-4489-947c-524743b68a2c"},"_deposit":{"created_by":12,"id":"2802","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2802"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002802","sets":["87:627:628:388:393"]},"author_link":["2571","6718"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"12","bibliographicPageStart":"7","bibliographicVolumeNumber":"70","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An inulin fructotransferase (DFA I-producing) [EC 2.4.1.200] from Arthrobacter sp.B69-5 was purified and the characters of the enzyme were investigated. The enzyme was purified from a culture supernatant of the microorganism by 9.41 fold with a yield of 17.9 % using a DEAE-Toyopearl chromatography and a Butyl-Toyopearl chromatography. The enzyme showed maximum activity at pH 5.5 and 45 ℃. The enzyme activity was stable up to 75 ℃. The molecular mass of the enzyme was estimated to be 40 kDa by SDS-PAGE and 42 kDa by gel filtration, and was considered to be a monomer. The N-terminal amino acid sequence (20 amino acid residues) was determined as ANTVYDVTTWSGATISPYVD.\n","subitem_description_type":"Abstract"},{"subitem_description":"多糖類イヌリンからオリゴ糖DFAⅠを生じる酵素の生産菌が新たに分離された. 分類学的な検討を行った結果, 本菌株はArthrobacter sp. B69-5と同定された. 本菌株が培養上清中に生産する酵素をイオン交換クロマト, 疎水クロマトにより電気泳動的に均一に精製した. 本酵素の反応至適pHは5.5, 反応至適温度は45℃であった. 20分間の加熱に対して, 本酵素は75℃まで安定であった. 本酵素の分子量についてはSDS-PAGEから40kDa, ゲル濾過から42kDaという数字が得られ単量体酵素であると推察された. 酵素蛋白のＮ-末端のアミノ酸配列を分析するとANTVYDVTTWSGATISPYVDという結果が得られた.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002685","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002685 ","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"原口, 和朋"},{"creatorName":"ハラグチ, カズトモ","creatorNameLang":"ja-Kana"},{"creatorName":"HARAGUCHI, Kazutomo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6718","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006426314","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006426314"},{"nameIdentifier":"90353961","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90353961"},{"nameIdentifier":"read0134913","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0134913"}]},{"creatorNames":[{"creatorName":"大坪, 研一"},{"creatorName":"オオツボ, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"Ohtsubo, Kenichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"2571","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80353960","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80353960"},{"nameIdentifier":"keno","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/keno"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-03"}],"displaytype":"detail","filename":"nfri_report_No70p7-12p.pdf","filesize":[{"value":"242.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No70p7-12p.pdf ","url":"https://repository.naro.go.jp/record/2802/files/nfri_report_No70p7-12p.pdf"},"version_id":"61a62711-0f19-4f21-8fef-8fc0f0e508dd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"inulin","subitem_subject_scheme":"Other"},{"subitem_subject":"DFAⅠ(difructose dianhydrideⅠ)","subitem_subject_scheme":"Other"},{"subitem_subject":"Arthrobacter","subitem_subject_scheme":"Other"},{"subitem_subject":"inulin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"DFAⅠ(difructose dianhydrideⅠ)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Arthrobacter","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Arthrobactersp. B69-5のInulin fructotransferase (DFA I-producing)の精製と性質","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Arthrobactersp. B69-5のInulin fructotransferase (DFA I-producing)の精製と性質"},{"subitem_title":"Purification and Properties of an Inulin Fructotransferase (DFA Ⅰ-Producing) from Arthrobacter sp. B69-5.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["393"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2802","relation_version_is_last":true,"title":["Arthrobactersp. B69-5のInulin fructotransferase (DFA I-producing)の精製と性質"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T23:04:21.348629+00:00"}