{"created":"2023-05-15T13:38:45.998756+00:00","id":2791,"links":{},"metadata":{"_buckets":{"deposit":"bdcfc764-6caf-481a-9568-7605858387d6"},"_deposit":{"created_by":12,"id":"2791","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2791"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002791","sets":["87:627:628:388:391"]},"author_link":["360","6797","6689","358"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"47","bibliographicPageStart":"43","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two types of the plasmid were prepared. One was the circular plasmid having the inverted repeat of two units of the telomeric repeat unit (5’-TTAGGGTCAACA) for Aspergillus oryzae and the autonomous replicating sequence AMA1 for Aspergillus nidulans. The other was the linear plasmid having two telomere units with both ends and AMA1 inside. Both plasmids were introduced in A. oryzae. The resulting transformants using the circular plasmid appeared to harbor the original circular one. The transformants with the linear plasmid harbored the circular one which was derived from the linear one by self-circularization. Therefore the two telomeric units were not recognized as the terminus of the DNA. More telomeric repeat units might be necessary for construction of the linear vector using the telomeric sequence of A. oryzae.\n","subitem_description_type":"Abstract"},{"subitem_description":"Aspergillus oryzaeのテロメア配列のうち, 繰り返し単位 (5'-TTAGGGTCAACA) 2回ずつを逆方向繰り返し配列として付加し, Aspergillus nidulansの自己複製配列AMA1を連結した環状プラスミドおよび繰り返し単位2回を両端に有し, 内部にAMA1を連結した線状プラスミドを作成した. これらのプラスミドをA. oryzaeに導入した結果, 環状プラスミドとして導入すると環状のまま保持され, 線状プラスミドの場合は自己閉環した. このことから, テロメア2回繰り返し配列は末端として認識されず, A. oryzaeのテロメア配列を利用して線状ベクターを作成するためにはさらに多くの繰り返し単位が必要と考えられた.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002674","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002674 ","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"KUSUMOTO, Kenichi","creatorNameLang":"en"},{"creatorName":"楠本, 憲一","creatorNameLang":"ja"},{"creatorName":"クスモト, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSUMOTO, Ken-ichi","creatorNameLang":"en"}],"familyNames":[{"familyName":"KUSUMOTO","familyNameLang":"en"},{"familyName":"楠本","familyNameLang":"ja"},{"familyName":"クスモト","familyNameLang":"ja-Kana"},{"familyName":"KUSUMOTO","familyNameLang":"en"}],"givenNames":[{"givenName":"Kenichi","givenNameLang":"en"},{"givenName":"憲一","givenNameLang":"ja"},{"givenName":"ケンイチ","givenNameLang":"ja-Kana"},{"givenName":"Ken-ichi","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"358","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80353978","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80353978"},{"nameIdentifier":"washokuuma","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/washokuuma"}]},{"creatorNames":[{"creatorName":"木村, 多江"},{"creatorName":"キムラ, タエ","creatorNameLang":"ja-Kana"},{"creatorName":"KIMURA, Tae","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6797","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000004555669","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000004555669"}]},{"creatorNames":[{"creatorName":"鈴木, 聡"},{"creatorName":"スズキ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"SUZUKI, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"360","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90353979","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90353979"},{"nameIdentifier":"fungaldevelopment","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/fungaldevelopment"}]},{"creatorNames":[{"creatorName":"柏木, 豊"},{"creatorName":"カシワギ, ユタカ","creatorNameLang":"ja-Kana"},{"creatorName":"KASHIWAGI, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6689","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000001832738","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000001832738"},{"nameIdentifier":"read0005775","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0005775"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-03"}],"displaytype":"detail","filename":"nfri_report_No68p43-47p.pdf","filesize":[{"value":"86.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No68p43-47p.pdf","url":"https://repository.naro.go.jp/record/2791/files/nfri_report_No68p43-47p.pdf"},"version_id":"0c598e8a-1706-4976-ba1b-a54b1a474859"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Aspergillus oryzaeのテロメア配列を付加したプラスミドの細胞内における形態の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Aspergillus oryzaeのテロメア配列を付加したプラスミドの細胞内における形態の解析"},{"subitem_title":"Structure of the plasmid with telomeric repeat sequences for Aspergillus oryzae","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["391"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2791","relation_version_is_last":true,"title":["Aspergillus oryzaeのテロメア配列を付加したプラスミドの細胞内における形態の解析"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-20T06:24:39.097010+00:00"}