{"created":"2023-05-15T13:38:45.866167+00:00","id":2789,"links":{},"metadata":{"_buckets":{"deposit":"c0a6c57f-da0a-4e4e-8e4e-5784b6579532"},"_deposit":{"created_by":12,"id":"2789","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2789"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002789","sets":["87:627:628:388:391"]},"author_link":["6689","358","360","6764","6753"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"37","bibliographicPageStart":"33","bibliographicVolumeNumber":"68","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A simple method for total RNA isolation from Aspergillus oryzae in solid-state culture (rice koji culture) was developed. A nucleic acid binding dye, aurintricarboxylic acid ammonium salt (ATA), was employed to prevent RNA from degradation by nuclease activity of rice koji culture. We also used cetyltrimethylammonium bromide (CTAB) to remove polysaccharides, which was mainly derived from rice grains. We successfully isolated total RNA from A. oryzae in solid-state culture. The RNA isolated by this method was available for Northern blot analysis and as a template of reverse transcription.\n","subitem_description_type":"Abstract"},{"subitem_description":"米麹からの麹菌全RNAの簡便な抽出法を開発した. 本方法は米麹の核酸分解活性からRNAを保護するためATAを用い, また多糖類の除去のためにCTABを用いる. 本方法により抽出されたRNAは, ノーザンブロット法及びRACE法等に供試可能であることを確認した\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002672","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002672 ","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 聡"},{"creatorName":"スズキ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"SUZUKI, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"竹谷, 博子"},{"creatorName":"タケタニ, ヒロコ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKETANI, Hiroko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"石田, 博樹"},{"creatorName":"イシダ, ヒロキ","creatorNameLang":"ja-Kana"},{"creatorName":"ISHIDA, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"KUSUMOTO, Kenichi","creatorNameLang":"en"},{"creatorName":"楠本, 憲一","creatorNameLang":"ja"},{"creatorName":"クスモト, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSUMOTO, Ken-ichi","creatorNameLang":"en"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"柏木, 豊"},{"creatorName":"カシワギ, ユタカ","creatorNameLang":"ja-Kana"},{"creatorName":"KASHIWAGI, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-04"}],"displaytype":"detail","filename":"nfri_report_No68p33-37p.pdf","filesize":[{"value":"78.5 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No68p33-37p.pdf","url":"https://repository.naro.go.jp/record/2789/files/nfri_report_No68p33-37p.pdf"},"version_id":"0551f0d7-a566-49eb-b6d5-3ed5375d0c42"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アウリントリカルボン酸アンモニウムを用いた米麹からの麹菌全RNA抽出法の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アウリントリカルボン酸アンモニウムを用いた米麹からの麹菌全RNA抽出法の検討"},{"subitem_title":"Method of total RNA isolation from Aspergillus oryzae in solid-state culture (rice koji culture) using Aurintricarboxylic Acid Ammonium Salt (ATA)","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["391"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2789","relation_version_is_last":true,"title":["アウリントリカルボン酸アンモニウムを用いた米麹からの麹菌全RNA抽出法の検討"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-20T06:24:38.823872+00:00"}