@article{oai:repository.naro.go.jp:00002789,
 author = {鈴木, 聡 and SUZUKI, Satoshi and 竹谷, 博子 and TAKETANI, Hiroko and 石田, 博樹 and ISHIDA, Hiroki and KUSUMOTO, Kenichi and 楠本, 憲一 and KUSUMOTO, Ken-ichi and 柏木, 豊 and KASHIWAGI, Yutaka},
 journal = {食品総合研究所研究報告, Report of National Food Research Institute},
 month = {Mar},
 note = {A simple method for total RNA isolation from Aspergillus oryzae in solid-state culture (rice koji culture) was developed. A nucleic acid binding dye, aurintricarboxylic acid ammonium salt (ATA), was employed to prevent RNA from degradation by nuclease activity of rice koji culture. We also used cetyltrimethylammonium bromide (CTAB) to remove polysaccharides, which was mainly derived from rice grains. We successfully isolated total RNA from A. oryzae in solid-state culture. The RNA isolated by this method was available for Northern blot analysis and as a template of reverse transcription., 米麹からの麹菌全RNAの簡便な抽出法を開発した. 本方法は米麹の核酸分解活性からRNAを保護するためATAを用い, また多糖類の除去のためにCTABを用いる. 本方法により抽出されたRNAは, ノーザンブロット法及びRACE法等に供試可能であることを確認した},
 pages = {33--37},
 title = {アウリントリカルボン酸アンモニウムを用いた米麹からの麹菌全RNA抽出法の検討},
 volume = {68},
 year = {2004},
 yomi = {スズキ, サトシ and タケタニ, ヒロコ and イシダ, ヒロキ and クスモト, ケンイチ and カシワギ, ユタカ}
}