{"created":"2023-05-15T13:38:45.219216+00:00","id":2779,"links":{},"metadata":{"_buckets":{"deposit":"1060a664-7834-4c56-b481-221dcfa34e68"},"_deposit":{"created_by":12,"id":"2779","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2779"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002779","sets":["87:627:628:388:390"]},"author_link":["360","6689","358"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"38","bibliographicPageStart":"33","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A gene trap vector for Aspergillus oryzae was constructed for use in comprehensive analysis of gene function. This vector, pPTR-EGFP1, has the egfp gene as a reporter gene preceded by a splice acceptor sequence, followed by A. nidulans sC gene terminator and pyrithiamine resistant gene (ptrA) as a selective marker in A.oryzae. This vector was integrated into a host genome randomly to create tag-line transformants. Approximately 300 pyrithiamine-resistant transformants were thus obtained, one of which trapped the 5' end of the coding region of yeast DUR3 homologue. Our results showed that pPTR-EGFP1 could be a useful tool in the gene function analysis of A.oryzae, namely, by isolating unknown genes by plasmid rescue and by trapping the endogenous promoter activity.\n","subitem_description_type":"Abstract"},{"subitem_description":"麹菌Aspergillus oryzaeの包括的な遺伝子機能解析に資するため、新規のジーントラップ遺伝子破壊用プラスミドベクターを開発した。このベクター、pPTR-EGFPlはスプライス受容保存配列とA.nidulansのsC遺伝子ターミネーター配列を付加した緑色蛍光タンパク質遣伝子をレポーター遺伝子として保持し、麹菌で働く選抜マーカー遺伝子としてピリチアミン耐性遺伝子(ptrA) を保持する。このベクターを宿主麹菌のゲノムDNA内にランダムに挿入することにより、タグライン形質転換株を作成することができる。このベクターを用いた形質転換実験により、約300株のピリチアミン耐性株が得られ、そのうちの1株が酵母の尿素運搬体タンパク遺伝子に相同な遺伝子のコード領域の5'末をトラップしていた。本ベクターpPTR-EGFP1を用いることにより、形質転換株からのプラスミドレスキュー法による未知遺伝子の回収、及び内在性のプロモーター活性のトラップが可能となることが示され、麹菌A.oryzaeの遺伝子機能解析においてpPTR-EGFP1が有効に機能することが明らかとなった。\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002662","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002662 ","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 聡"},{"creatorName":"スズキ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"SUZUKI, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"KUSUMOTO, Kenichi","creatorNameLang":"en"},{"creatorName":"楠本, 憲一","creatorNameLang":"ja"},{"creatorName":"クスモト, ケンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"KUSUMOTO, Ken-ichi","creatorNameLang":"en"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"柏木, 豊"},{"creatorName":"カシワギ, ユタカ","creatorNameLang":"ja-Kana"},{"creatorName":"KASHIWAGI, Yutaka","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-03"}],"displaytype":"detail","filename":"nfri_report_No67p33-38p.pdf","filesize":[{"value":"518.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No67p33-38p.pdf ","url":"https://repository.naro.go.jp/record/2779/files/nfri_report_No67p33-38p.pdf"},"version_id":"ae436f4b-e470-42e1-bf00-470aeacf409a"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"麹菌用ジーントラップベクター, pPTR-EGFP1の作成","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"麹菌用ジーントラップベクター, pPTR-EGFP1の作成"},{"subitem_title":"Construction of a gene trap vector, pPTR-EGFP1, for the filamentous fungus, Aspergillus oryzae","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["390"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2779","relation_version_is_last":true,"title":["麹菌用ジーントラップベクター, pPTR-EGFP1の作成"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-20T06:24:44.483668+00:00"}