{"created":"2023-05-15T13:38:45.153816+00:00","id":2778,"links":{},"metadata":{"_buckets":{"deposit":"d876c667-4a37-47fd-9197-023b039179e0"},"_deposit":{"created_by":12,"id":"2778","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2778"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002778","sets":["87:627:628:388:390"]},"author_link":["1474","6738","6728"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"31","bibliographicPageStart":"27","bibliographicVolumeNumber":"67","bibliographic_titles":[{"bibliographic_title":"食品総合研究所研究報告"},{"bibliographic_title":"Report of National Food Research Institute","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Acrylamide is a potential cancer-causing agent, and it is present in fried and oven-cooked foods. We evaluated DNA-damaging act1vity of acrylamide using comet (single-cell gel electrophoresis) assay. HL60 (human promyelocytic leukemic cell line, 1x10^6 cells/ml) were treated with acrylamide at various concentrations such as 1, 10, 100, 1000, 10000ppm for 30 min in a CO_2 incubator (5% CO_2, 95% air, 37°C). Among 6 indexes of comet assay including tail length, shape factor, nuclear diameter, tail intensity, tail moment, and DNA migration, tail moment properly reflected DNA damage of HL60 cell by acrylamide. In every case more than 95% of cells were alive after 30 min incubation. Two hour treatment of HL60 with acrylamide (0.01-10 ppm) brought highly sensitive detection of the activity with a dose dependent manner.\n","subitem_description_type":"Abstract"},{"subitem_description":"コメットアッセイ(smgle-cell gel electrophoresis) を用いて, 食品の加熱中にアミノカルボニル反応により生成し, 発がん性が疑われている化合物, アクリルアミドのDNA損傷作用を調べた. ヒト由来HL60細胞とアクリルアミドを30分間培養したとき, コメットアッセイの6つのインテツクスはアクリルアミド濃度が1-10000ppmまで濃度に依存して増加した. tail momentをインデックスとして検出感度を上げるための条件を検討した結果, 2時間の培養でアクリルアミドのDNA損傷作用は, 0.1～10 ppmまで濃度依存的に増加し, 1 ppm以上で有意差が認められた (P<0.001) .\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002661","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002661 ","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0301-9780","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"三輪, 操"},{"creatorName":"ミワ, ミサオ","creatorNameLang":"ja-Kana"},{"creatorName":"MIWA, Misao","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6728","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000020015435","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000020015435"},{"nameIdentifier":"read0117227","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0117227"}]},{"creatorNames":[{"creatorName":"松丸, 裕之"},{"creatorName":"マツマル, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"MATSUMARU, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6738","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000009720168","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000009720168"}]},{"creatorNames":[{"creatorName":"内藤, 成弘"},{"creatorName":"ナイトウ, シゲヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"NAITO, Shigehiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1474","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"30353999","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=30353999"},{"nameIdentifier":"s_naito","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/s_naito"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-10-03"}],"displaytype":"detail","filename":"nfri_report_No67p27-31p.pdf","filesize":[{"value":"465.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nfri_report_No67p27-31p.pdf ","url":"https://repository.naro.go.jp/record/2778/files/nfri_report_No67p27-31p.pdf"},"version_id":"91b750d8-4f3e-4af9-a010-0cfca48464a1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"コメットアッセイを用いたアクリルアミドのDNA損傷作用の評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"コメットアッセイを用いたアクリルアミドのDNA損傷作用の評価"},{"subitem_title":"DNA-damaging Activity of Acrylamide Evaluated by Comet Assay","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["390"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-12-20"},"publish_date":"2019-12-20","publish_status":"0","recid":"2778","relation_version_is_last":true,"title":["コメットアッセイを用いたアクリルアミドのDNA損傷作用の評価"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T16:16:09.587955+00:00"}