{"created":"2023-05-15T13:38:35.007224+00:00","id":2554,"links":{},"metadata":{"_buckets":{"deposit":"511ac666-f96b-420a-9117-1c0c31db7b9a"},"_deposit":{"created_by":11,"id":"2554","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2554"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002554","sets":["2:581:116","87:551:679:51","87:654:46"]},"author_link":["1161","1489","1876","552","6527"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-03","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"219","bibliographicPageStart":"211","bibliographicVolumeNumber":"147","bibliographic_titles":[{"bibliographic_title":"Phytochemistry"},{"bibliographic_title":"Phytochemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"MLX56 family defense proteins, MLX56 and its close homolog LA-b, are chitin-binding defense proteins found in mulberry latex that show strong growth-inhibitions against caterpillars when fed at concentrations as low as 0.01%. MLX56 family proteins contain a unique structure with an extensin domain surrounded by two hevein-like chitin-binding domains, but their defensive modes of action remain unclear. Here, we analyzed the effects of MLX56 family proteins on the peritrophic membrane (PM), a thin and soft membrane consisting of chitin that lines the midgut lumen of insects. We observed an abnormally thick (>1/5 the diameter of midgut) hard gel-like membrane consisted of chitin and MLX56 family proteins, MLX56 and LA-b, in the midgut of the Eri silkworms, Samia ricini, fed a diet containing MLX56 family proteins, MLX56 and LA-b. When polyoxin AL, a chitin-synthesis-inhibitor, was added to the diet containing MLX56 family proteins, the toxicity of MLX56 family proteins disappeared and PM became thinner and fragmented. These results suggest that MLX56 family proteins, through their chitin-binding domains, bind to the chitin framework of PM, then through their extensin-domain (gum arabic-like structure), which functions as swelling agent, expands PM into an abnormally thick membrane that inhibits the growth of insects. This study shows that MLX56 family proteins are plant defense lectins with a totally unique mode of action, and reveals the functions of extensin domains and arabinogalactan proteins as swelling (gel-forming) agents of plants.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.phytochem.2018.01.005","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC30201804040024"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"author manuscript "}]},{"subitem_relation_name":[{"subitem_relation_name_text":"この論文は出版社版ではありません。引用の際には出版社版をご利用ください。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":" This is not the published version. Please cite only the published version. "}]},{"subitem_relation_name":[{"subitem_relation_name_text":"Green open Access /This journal has an embargo period of 24 months."}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Phytochemistry"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.phytochem.2018.01.005","subitem_relation_type_select":"DOI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The Author(s) "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0031-9422","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"今野, 浩太郎"},{"creatorName":"コンノ, コウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"KOMNO, Kotaro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"志村, 幸子"},{"creatorName":"シムラ, サチコ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIMURA, Sachiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"UENO, Chihiro"},{"creatorName":"ウエノ, チヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"UENO, Chihiro","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"新川, 徹"},{"creatorName":"アラカワ, トオル","creatorNameLang":"ja-Kana"},{"creatorName":"ARAKAWA, Toru","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"中村, 匡利"},{"creatorName":"ナカムラ, マサトシ","creatorNameLang":"ja-Kana"},{"creatorName":"NAKAMURA, Masatoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-04-01"}],"displaytype":"detail","filename":"SC30201804040024_postprint-a.pdf","filesize":[{"value":"5.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC30201804040024_postprint.pdf","url":"https://repository.naro.go.jp/record/2554/files/SC30201804040024_postprint-a.pdf"},"version_id":"83ac2b8f-456c-499e-b299-960221f599dd"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Morus albaMoraceae","subitem_subject_scheme":"Other"},{"subitem_subject":"MLX56 family proteins","subitem_subject_scheme":"Other"},{"subitem_subject":"Arabinogalactan protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Chitin-binding lectin","subitem_subject_scheme":"Other"},{"subitem_subject":"Extensin domain","subitem_subject_scheme":"Other"},{"subitem_subject":"Plant latex defense protein","subitem_subject_scheme":"Other"},{"subitem_subject":"Peritrophic membrane","subitem_subject_scheme":"Other"},{"subitem_subject":"Plant-insect interactionSwelling (gel-forming) agent","subitem_subject_scheme":"Other"},{"subitem_subject":"Morus albaMoraceae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"MLX56 family proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Arabinogalactan protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Chitin-binding lectin","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Extensin domain","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Plant latex defense protein","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Peritrophic membrane","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Plant-insect interactionSwelling (gel-forming) agent","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Abnormal swelling of the peritrophic membrane in Eri silkworm gut caused by MLX56 family defense proteins with chitin-binding and extensin domains","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Abnormal swelling of the peritrophic membrane in Eri silkworm gut caused by MLX56 family defense proteins with chitin-binding and extensin domains"},{"subitem_title":"Abnormal swelling of the peritrophic membrane in Eri silkworm gut caused by MLX56 family defense proteins with chitin-binding and extensin domains","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["46","51","116"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-05-14"},"publish_date":"2019-05-14","publish_status":"0","recid":"2554","relation_version_is_last":true,"title":["Abnormal swelling of the peritrophic membrane in Eri silkworm gut caused by MLX56 family defense proteins with chitin-binding and extensin domains"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T15:38:28.307132+00:00"}