{"created":"2023-05-15T13:38:32.921088+00:00","id":2515,"links":{},"metadata":{"_buckets":{"deposit":"e4210123-c388-41f0-8e73-25cc066a8ad3"},"_deposit":{"created_by":11,"id":"2515","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2515"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002515","sets":["2:581:109","87:627:45"]},"author_link":["953","1037","1657"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-06-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"396","bibliographicPageStart":"390","bibliographicVolumeNumber":"252","bibliographic_titles":[{"bibliographic_title":"Food Chemistry"},{"bibliographic_title":"Food Chemistry","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We developed new loop-mediated isothermal amplification (LAMP)-based detection methods for the screening of genetically modified (GM) maize and soybean events. The LAMP methods developed targeted seven sequences: cauliflower mosaic virus 35S promoter; 5-enolpyruvylshikimate-3-phosphate synthase gene from Agrobacterium tumefaciens strain CP4 (cp4epsps); phosphinothricin acetyltransferase (pat) gene; mannose-6-phosphate isomerase gene; Pisum sativum ribulose 1, 5-bisphosphate carboxylase terminator; a common sequence between Cry1Ab and Cry1Ac genes; and a GA21 construct-specific sequence. We designed new specific primer sets for each target, and the limit of detection (LOD) was evaluated using authorized GM maize and soybean events. LODs for each target were ≤ 0.5%. To make the DNA extraction process simple and rapid, we also developed a direct LAMP detection scheme using crude cell lysates. The entire process, including pretreatments and detection, could be completed within 1 h.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.foodchem.2017.12.036","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC30201803130001"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"Green open Access"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This journal has an embargo period of 12 months."}]},{"subitem_relation_name":[{"subitem_relation_name_text":"この論文は出版社版ではありません。引用の際には出版社版をご利用ください。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This is not the published version. Please cite only the published version. "}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Food Chemistry"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.foodchem.2017.12.036","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"author manuscript"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"2017 Published by Elsevier Ltd. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0308-8146","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"高畠, 令王奈"},{"creatorName":"タカバタケ, レオナ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKABATAKE, Reona","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1657","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20463466","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20463466"},{"nameIdentifier":"Takabataker","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/Takabataker"},{"nameIdentifier":"0000-0001-6419-7314","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0001-6419-7314"}]},{"creatorNames":[{"creatorName":"真野, 潤一"},{"creatorName":"マノ, ジュンイチ","creatorNameLang":"ja-Kana"},{"creatorName":"MANO, Junichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"953","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"90442764","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=90442764"},{"nameIdentifier":"jmano","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/jmano"}]},{"creatorNames":[{"creatorName":"橘田, 和美"},{"creatorName":"キッタ, カズミ","creatorNameLang":"ja-Kana"},{"creatorName":"KITTA, Kazumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1037","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60353926","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60353926"},{"nameIdentifier":"FAAU","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/FAAU"},{"nameIdentifier":"0000-0002-3691-271X","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0002-3691-271X"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-07-01"}],"displaytype":"detail","filename":"SC30201803130001PostPrint.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC30201803130001PostPrint","url":"https://repository.naro.go.jp/record/2515/files/SC30201803130001PostPrint.pdf"},"version_id":"b57dd67e-b89f-47b2-a0a2-587c06fa36a0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Loop-mediated isothermal amplification (LAMP)","subitem_subject_scheme":"Other"},{"subitem_subject":"Genetically modified (GM)","subitem_subject_scheme":"Other"},{"subitem_subject":"Direct LAMP","subitem_subject_scheme":"Other"},{"subitem_subject":"Rapid qualitative analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"Loop-mediated isothermal amplification (LAMP)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Genetically modified (GM)","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Direct LAMP","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rapid qualitative analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Development and evaluation of rapid screening detection methods for genetically modified crops using loop-mediated isothermal amplification","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Development and evaluation of rapid screening detection methods for genetically modified crops using loop-mediated isothermal amplification"},{"subitem_title":"Development and evaluation of rapid screening detection methods for genetically modified crops using loop-mediated isothermal amplification","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["45","109"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-05-14"},"publish_date":"2019-05-14","publish_status":"0","recid":"2515","relation_version_is_last":true,"title":["Development and evaluation of rapid screening detection methods for genetically modified crops using loop-mediated isothermal amplification"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T15:44:01.286380+00:00"}