{"created":"2023-05-15T13:38:32.403083+00:00","id":2507,"links":{},"metadata":{"_buckets":{"deposit":"b9282047-aa3c-434c-8bf1-b2aedb54fdbd"},"_deposit":{"created_by":11,"id":"2507","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"2507"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002507","sets":["2:581:101","87:654:46"]},"author_link":["1093"],"item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"57","bibliographicPageStart":"50","bibliographicVolumeNumber":"77","bibliographic_titles":[{"bibliographic_title":"Cryobiology"},{"bibliographic_title":"Cryobiology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Trehalose is a promising natural cryoprotectant, but its cryoprotective effect is limited due to difficulties in transmembrane transport. Thus, expressing the trehalose transporter TRET1 on various mammalian cells may yield more trehalose applications. In this study, we ran comparative cryopreservation experiments between the TRET1-expressing cells and the wild-type cells under various trehalose concentrations in an extracellular medium and under various freezing temperatures. We show here that the intracellular trehalose significantly improves the viability of freeze-thawing cells. The optimum trehalose concentration is about 400 mM, and the optimum freezing temperature ranges between 140 and 240 K. We find a qualitative coincidence of the optimum freezing temperature with that from including 10 vol% dimethyl sulfoxide, a result that indicates that both intra- and extracellular trehalose principally work to inhibit ice crystal growth and thus protect the membrane and proteins from ice crystals during relatively slow freezing.","subitem_description_type":"Abstract"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.cryobiol.2017.05.008","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_16":{"attribute_name":"情報源","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"SC30201804090007"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"NARO成果DBa"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"Green open Access"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This journal has an embargo period of 12 months."}]},{"subitem_relation_name":[{"subitem_relation_name_text":" この論文は出版社版ではありません。引用の際には出版社版をご利用ください。"}]},{"subitem_relation_name":[{"subitem_relation_name_text":"This is not the published version. Please cite only the published version. "}]}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"Cryobiology"}],"subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.cryobiol.2017.05.008","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_18":{"attribute_name":"他の資源との関係","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_text":"author manuscript"}]}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"2017 Published by Elsevier Ltd. "}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0011-2240","subitem_source_identifier_type":"ISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"黄川田, 隆洋"},{"creatorName":"キカワダ, タカヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"KIKAWADA, Takahiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1093","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"60414900","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=60414900"},{"nameIdentifier":"kikawada","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/kikawada"},{"nameIdentifier":"0000-0002-2329-9282","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0002-2329-9282"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-01"}],"displaytype":"detail","filename":"SC30201804090007_PostPrint.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"SC30201804090007_PostPrint","url":"https://repository.naro.go.jp/record/2507/files/SC30201804090007_PostPrint.pdf"},"version_id":"468eff2d-83c4-4ce0-adbb-927d895c59a2"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"trehalose","subitem_subject_scheme":"Other"},{"subitem_subject":"trehalose transporter 1","subitem_subject_scheme":"Other"},{"subitem_subject":"cryoprotectant","subitem_subject_scheme":"Other"},{"subitem_subject":"freezing temperature","subitem_subject_scheme":"Other"},{"subitem_subject":"trehalose concentration","subitem_subject_scheme":"Other"},{"subitem_subject":"CHO-K1 cell","subitem_subject_scheme":"Other"},{"subitem_subject":"trehalose","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"trehalose transporter 1","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cryoprotectant","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"freezing temperature","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"trehalose concentration","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"CHO-K1 cell","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Intracellular trehalose via transporter TRET1 as a method to cryoprotect CHO-K1 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Intracellular trehalose via transporter TRET1 as a method to cryoprotect CHO-K1 cells"},{"subitem_title":"Intracellular trehalose via transporter TRET1 as a method to cryoprotect CHO-K1 cells","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"11","path":["46","101"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-05-14"},"publish_date":"2019-05-14","publish_status":"0","recid":"2507","relation_version_is_last":true,"title":["Intracellular trehalose via transporter TRET1 as a method to cryoprotect CHO-K1 cells"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2023-05-15T16:23:42.602316+00:00"}