{"created":"2023-05-15T13:36:36.610978+00:00","id":24,"links":{},"metadata":{"_buckets":{"deposit":"39825009-87f9-4408-a293-587cf93c1b25"},"_deposit":{"created_by":11,"id":"24","owners":[11],"pid":{"revision_id":0,"type":"depid","value":"24"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00000024","sets":["87:597:36","87:597:592:10:24"]},"author_link":["92","91","93","88","87","94","89","90"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"119","bibliographicPageEnd":"106","bibliographicPageStart":"89","bibliographic_titles":[{"bibliographic_title":"農研機構研究報告 東北農業研究センター"},{"bibliographic_title":"Bulletin of the NARO, Agricultural Research for Tohoku Region","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"東北地域のダイズの収量安定化のため、主要病虫害であるダイズシストセンチュウ（SCN）、ダイズモザイクウイルス（SMV）、ダイズわい化ウイルス（SbDV）に対する抵抗性の付与は重要である。本研究では抵抗性遺伝子近傍から複数の単純反復配列（SSR）マーカーセットを選定し、DNAシーケンサーのフラグメント解析により複数マーカーを同時に解析することでマーカー選抜を効率化し、戻し交配により「リュウホウ」及び「おおすず」を反復親として、各々、SCN及びSMV抵抗性を導入した系統「東北173号」、SMV及びSbDV抵抗性を導入した系統「東北174号」等を開発した。生物検定の結果、SCNに対して感受性を示す系統が一部存在し、SCN抵抗性マーカーセットは改良の余地があるが、SMV及びSbDVに対して、マーカーにより選抜した系統はいずれも抵抗性が確認され、マーカー\nの有効性が示された。育成系統と反復親との農業形質を比較したところ、「東北173号」は「リュウホウ」とほぼ同等であったが、「東北174号」は多くの形質で「おおすず」と有意な差が認められ、導入目的の遺伝子以外のゲノム領域が「東北174号」により多く残存した可能性が考えられた。これらのことから、本マーカー選抜手法は有効であり、さらに、これら育成系統は抵抗性の育種母本として有望と考えられる。","subitem_description_type":"Abstract"},{"subitem_description":"In order to stabilize soybean yield in the Tohoku district, it is important to breed cultivars with resistances to soybean cyst nematode（SCN），soybean mosaic virus（SMV），and soybean dwarf virus（SbDV）．In this study, we selected a series of simple sequence repeat（SSR）marker sets located near each resistance locus, and simultaneously analyzed them with a high-resolution polymerase chain reaction（PCR）fragment analysis system to promote efficiency in marker-assisted selection（MAS）．\nWith backcrossing, our‘Tohoku 173’ upbringing system was developed by introducing the SCN and SMV resistance genes to‘Ryuhou,’ while‘Tohoku 174’ was developed by introducing the SMV and SbDV resistance genes to‘Ohsuzu.’ Inoculation tests showed that a marker set for the selection of SCN needed to be improved since a breeding line selected by MAS showed susceptibility, while the\nmarker sets for SMV and SbDV were found to be valid since all lines selected by MAS showed resistance to both viral diseases. A comparison of the agricultural characteristics of the developed lines with those of recurrent parents showed that the characteristics of‘Tohoku 173’ and‘Ryuhou’ were nearly identical to each  Other. However, there were many significant differences between\n‘Tohoku 174’ and‘Ohsuzu,’ indicating that‘Tohoku 174’ contains many genomic regions that originated from resistant parents. In conclusion, it was demonstrated that these marker-assisted selection systems are valid, and that the developed lines are desirable materials for breeding new cultivars with resistance to SCN, SMV, and SbDV.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00000015","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構"},{"subitem_publisher":"National Agriculture and Food Research Organization (NARO)"}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"2432-8081","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 信"},{"creatorName":"カトウ, シン","creatorNameLang":"ja-Kana"},{"creatorName":"KATO, Shin","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"佐山, 貴司"},{"creatorName":"サヤマ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"SAYAMA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"TAKADA, Yoshitake","creatorNameLang":"en"},{"creatorName":"高田, 吉丈","creatorNameLang":"ja"},{"creatorName":"タカダ, ヨシタケ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"湯本, 節三"},{"creatorName":"ユモト, セツゾウ","creatorNameLang":"ja-Kana"},{"creatorName":"YUMOTO, Setsuzo","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"石本, 政男"},{"creatorName":"イシモト, マサオ","creatorNameLang":"ja-Kana"},{"creatorName":"ISHIMOTO, Masao","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"島村, 聡"},{"creatorName":"シマムラ, サトシ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIMAMURA, Satoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"平田, 香里"},{"creatorName":"ヒラタ, カオリ","creatorNameLang":"ja-Kana"},{"creatorName":"HIRATA, Kaori","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"菊池, 彰夫"},{"creatorName":"キクチ, アキオ","creatorNameLang":"ja-Kana"},{"creatorName":"KIKUCHI, Akio","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-08-17"}],"displaytype":"detail","filename":"tarc_report119_8p.pdf","filesize":[{"value":"1.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"tarc_report119_8p.pdf","url":"https://repository.naro.go.jp/record/24/files/tarc_report119_8p.pdf"},"version_id":"ce978098-1a74-4243-8c87-369876ca09e1"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"ダイズ","subitem_subject_scheme":"Other"},{"subitem_subject":"ダイズシストセンチュウ","subitem_subject_scheme":"Other"},{"subitem_subject":"ダイズモザイクウイルス","subitem_subject_scheme":"Other"},{"subitem_subject":"ダイズわい化ウイルス","subitem_subject_scheme":"Other"},{"subitem_subject":"東 北地域","subitem_subject_scheme":"Other"},{"subitem_subject":"マーカー選抜","subitem_subject_scheme":"Other"},{"subitem_subject":"Soybean","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Soybean cyst nematode","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Soybean mosaic virus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Soybean dwarf virus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tohoku region","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Marker-assisted selection","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"シーケンサーのフラグメント解析を利用した DNAマーカー選抜による複合病虫害抵抗性ダイズ系統の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"シーケンサーのフラグメント解析を利用した DNAマーカー選抜による複合病虫害抵抗性ダイズ系統の開発"},{"subitem_title":"Breeding of Soybean Lines Conferred Multiple Disease and Pest Resistance by Marker-Assisted Selection with a High-Resolution PCR Fragment Analysis System","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"11","path":["24","36"],"pubdate":{"attribute_name":"公開日","attribute_value":"2017-08-17"},"publish_date":"2017-08-17","publish_status":"0","recid":"24","relation_version_is_last":true,"title":["シーケンサーのフラグメント解析を利用した DNAマーカー選抜による複合病虫害抵抗性ダイズ系統の開発"],"weko_creator_id":"11","weko_shared_id":-1},"updated":"2024-04-23T07:58:04.504850+00:00"}