{"created":"2023-05-15T13:38:25.625976+00:00","id":2365,"links":{},"metadata":{"_buckets":{"deposit":"2ac83a7d-aca0-4742-9622-46215ed7bb05"},"_deposit":{"created_by":12,"id":"2365","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2365"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002365","sets":["87:639:640:134:338"]},"author_link":["2165","3643","6292","6293","1810","2246"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-02-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"52","bibliographicPageStart":"47","bibliographicVolumeNumber":"119","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Akabane virus (AKAV), Aino virus (AINOV) and Peaton virus (PEAV) of the family Bunyaviridae, the genus Orthobunyavirus have been confirmed as etiological agents of bovine abnormal deliveries, such as abortion, stillbirth, premature birth and calf deformities. The outbreaks caused by these viruses have been observed in Japan and severely affected Japanese livestock industry. Furthermore, Shamonda virus (SHAV) and Sathuperi virus (SATV) of the genus Orthobunyavirus have been isolated repeatedly in recent years in Japan, and are suspected causes of bovine abnormal deliveries. In this study, we have developed a new RT-PCR assay for easy and rapid detection of the above mentioned 5 orthobunyaviruses. All of 15 AKAV, 4 AINOV, 5 PEAV, 3 SHAV and 4SATV isolates were detected by the technique. The detection sensitivity was different in each isolates and was ranging from 10 to 1,000 TCID_50/ml. These data indicate that our RT-PCR assay with the single primer set is useful for the detection of orthobunyaviruses which have been　isolated in Japan.\n","subitem_description_type":"Abstract"},{"subitem_description":"ブニャウイルス科オルソブニャウイルス属のアカバネウイルス(AKAV)やアイノウイルス(AINOV), ピートンウイルス(PEAV)による牛の流産, 死産, 早産, 先天異常子の出産といった異常産が日本ではたびたび流行し, 甚大な経済被害をもたらしている. さらに近年では,  これまで国内で確認されていなかったオルソブニャウイルス属のシャモンダウイルス(SHAV)やサシュペリウイルス(SATV)が相次いで分離され,  これらのウイルスの関与が疑われる牛の異常産の発生も報告されている. そのため, 我々は既報をもとに, SHAV, SATVを含む国内で分離されたオルソブニャウイルスを検出するRT-PCR法の改良を行い, その有用性を検証した. 国内外で分離されたAKAV15株,  AINOV4株,  PEAV5株,  SHAV3株,  SATV4株の培養上清から抽出したウイルスRNAを用いて検証実験を行ったところ, すべての株で特異遺伝子が検出された. 検出感度はウイルス株によって差がみられたが, ウイルス感染価10-1, 000 TCID_50/ml程度から検出可能であったことから, これらのオルソブニャウイルスの検出に有用であることが確認された. \n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002269","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002269","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"加藤, 友子"},{"creatorName":"カトウ, トモコ","creatorNameLang":"ja-Kana"},{"creatorName":"KATO, Tomoko","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"松本, 春菜"},{"creatorName":"マツモト, ハルナ","creatorNameLang":"ja-Kana"},{"creatorName":"MATSUMOTO, Haruna","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"平島, 宣昌"},{"creatorName":"ヒラシマ, ヨシマサ","creatorNameLang":"ja-Kana"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"白藤, 浩明"},{"creatorName":"シラフジ, ヒロアキ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIRAFUJI, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"山川, 睦"},{"creatorName":"ヤマカワ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"YAMAKAWA, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"梁瀬, 徹"},{"creatorName":"ヤナセ, トオル","creatorNameLang":"ja-Kana"},{"creatorName":"YANASE, Tohru","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-20"}],"displaytype":"detail","filename":"niah_report_No119p47-52p.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No119p47-52p.pdf","url":"https://repository.naro.go.jp/record/2365/files/niah_report_No119p47-52p.pdf"},"version_id":"d7df64f1-2cb7-452a-b964-1b85e61c655d"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"国内で分離されたオルソブニャウイルスを検出するための RT-PCR 法の改良","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"国内で分離されたオルソブニャウイルスを検出するための RT-PCR 法の改良"},{"subitem_title":"Improvement of RT-PCR assay for detection of orthobunyaviruses isolated in Japan","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["338"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"2365","relation_version_is_last":true,"title":["国内で分離されたオルソブニャウイルスを検出するための RT-PCR 法の改良"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T23:11:00.216083+00:00"}