{"created":"2023-05-15T13:38:22.132707+00:00","id":2302,"links":{},"metadata":{"_buckets":{"deposit":"31f1719c-8fb1-4b09-9150-1d90e8e617db"},"_deposit":{"created_by":12,"id":"2302","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2302"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002302","sets":["87:639:640:134:336"]},"author_link":["1810","1198","1367","5956","5979","1116"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-02-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"61","bibliographicPageStart":"55","bibliographicVolumeNumber":"117","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two different enzyme-linked immunosorbent assays (ELISAs) and real time PCR were developed as the new diagnostic tests of Caprine arthritis-encephalitis virus (CAEV) infection. The ELISAs used the whole virus (wCAEV) and recombinant protein of major core protein of CAEV (rPr55gag) as the antigen, and were designated wELISA and rELISA, respectively. The performance of each ELISA was evaluated by the western blot analysis using wCAEV as antigen (wWB). wELISA had substantially low sensitivity (80.4%) and specificity (78.0%) compared to wWB, and rELISA had lower sensitivity (78.2%) and specificity (61.1%) than wELISA. The lack of adequate sensitivity and specificity for rELISA and wELISA suggests that these assays need considerable modification. However, the WB used rPr55gag as antigen showed excellent agreement with wWB and that it can be used as a confirmatory test for the presence of anti-CAEV antibodies. The monoclonal and polyclonal antibodies against wCAEV and rPr55gag were tested by indirect immunofluorescence assay (IFA) and immunoperoxidase technique. The results of these assays suggested that the antibodies would be available for CAEV detection. The real time PCR had similar sensitivity to the preexisting nested PCR.\n","subitem_description_type":"Abstract"},{"subitem_description":"山羊関節炎・脳脊髄炎 （CAE） の新たな診断法として, ELISA 法およびリアルタイムPCR 法の開発を試みた. 精製ウイルス （wCAEV） と大腸菌組換え蛋白質（rPr55gag）を抗原とした二種類のELISA 法 （wELISA およびrELISA） を開発し, その精度をwCAEV を抗原としたウエスタンブロット法 （wWB） による野外血清745 検体の検査結果を基に分析した. wWB に対するwELISA の感度・特異度は80.4% および78.0% であり, rELISA の感度・特異度は78.2% および61.1% と低かった. しかし, rPr55gag を抗原としたrWB の感度・特異度は, 93.0% および96.3% と現行のCAEV診断法である寒天ゲル内沈降試験と同等の精度を有することが分かった. このことからrWB は, 抗原調製に時間を要するwWB に代わるCAEV の確定診断法として利用可能と考えられる. また, 各抗原を用いて単クローン抗体およびウサギ免疫血清を作製した. 各抗体は間接蛍光抗体法もしくは酵素抗体法において抗原と特異的な反応を示したことから, 病性鑑定等における抗原検出に利用可能と考えられる. さらに, CAEV のコア蛋白遺伝子を標的としたリアルタイムPCR 法は, 現行のnested PCR法と同等の感度を有しており, 多検体の検査における活用が期待される. \n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002206","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002206","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小西, 美佐子"},{"creatorName":"コニシ, ミサコ","creatorNameLang":"ja-Kana"},{"creatorName":"KONISHI, Misako","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"白藤, 浩明"},{"creatorName":"シラフジ, ヒロアキ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIRAFUJI, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"木村, 久美子"},{"creatorName":"キムラ, クミコ","creatorNameLang":"ja-Kana"},{"creatorName":"KIMURA, Kumiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"播谷, 亮"},{"creatorName":"ハリタニ, マコト","creatorNameLang":"ja-Kana"},{"creatorName":"HARITANI, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"KAMEYAMA, Kenichiro","creatorNameLang":"en"},{"creatorName":"KAMEYAMA, Ken-ichiro","creatorNameLang":"en"},{"creatorName":"亀山, 健一郎","creatorNameLang":"ja"},{"creatorName":"カメヤマ, ケンイチロウ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"村上, 賢二"},{"creatorName":"ムラカミ, ケンジ","creatorNameLang":"ja-Kana"},{"creatorName":"MURAKAMI, Kenji","creatorNameLang":"en"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-20"}],"displaytype":"detail","filename":"niah_report_No117p55-61p.pdf","filesize":[{"value":"375.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No117p55-61p.pdf ","url":"https://repository.naro.go.jp/record/2302/files/niah_report_No117p55-61p.pdf"},"version_id":"503cb1fd-70d1-4623-920a-08d25024ddf4"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"山羊関節炎・脳脊髄炎の新しい診断法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"山羊関節炎・脳脊髄炎の新しい診断法の開発"},{"subitem_title":"Development of novel diagnostic methods of Caprine arthritis-encephalitis virus infection","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["336"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"2302","relation_version_is_last":true,"title":["山羊関節炎・脳脊髄炎の新しい診断法の開発"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-20T07:53:47.474691+00:00"}