@article{oai:repository.naro.go.jp:00002256,
 author = {菅野, 徹 and KANNO, Toru and 石原, 涼子 and ISHIHARA, Ryouko and 畠間, 真一 and HATAMA, Shinichi and 内田, 郁夫 and UCHIDA, Ikuo and 山岸, 麻衣子 and YAMAGISHI, Maiko and 石川, 義春 and ISHIKAWA, Yoshiharu and 門田, 耕一 and KADOTA, Koichi},
 journal = {動物衛生研究所研究報告, Bulletin of the National Institute of Animal Health},
 month = {Feb},
 note = {Recent Japanese field isolates of bovine coronaviruses, reference Kakegawa strain and vaccine strain, which belongs to 4 genotypes by phylogenetic analysis of the S glycoprotein gene, were analyzed for their antigenic properties by indirect immunofluorescent and neutralization tests. In the results of neutralization tests, there are antigenic differences among viruses, however it didn’t show the degree of indication of distinct serotype. Monoclonal antibody, 4H4, against the Kakegawa strain, which belong to genotype 1, significantly lacked neutralizing activity for genotype 2, 3, and 4 viruses. Analysis of using escape mutant viruses from 4H4 revealed that the antigenic site of Kakegawa strain was mapped to at amino acid position 284 of S glycoprotein. Experimental infection of Kakegawa and Kumamoto strains were conducted for verification of their pathogenicity. The cattle and calves inoculated with the Kumamoto strain showed pyrexia and diarrhea, and viral gene was detected in plasma, nasal discharge and feces by RT-PCR. Histological examination showed lesions in small and large intestines mainly in ileum. BCoV RNA and antigen were detected in nasal turbinates, trachea, lung, rumen, reticulum, omasum and abomasum as well as intestinal tract., 牛コロナウイルスの国内流行株の抗原性比較解析を行った. ４つの遺伝子型に属すウイルス株間には中和抗原性に差が認められたが, 血清型の相違を示す程度ではなかった. 遺伝子型１に属す掛川株に対する中和モノクローナル抗体4H4 は, ２, ３および４型ウイルスに対する中和能が大きく低下していた. 4H4 に対する中和抵抗性変異株の解析により中和抗原部位がS 蛋白の284 番目のアミノ酸であることを特定した. 掛川株および熊本株を牛に経口接種し, 病原性再現試験を行った. 熊本株を接種した牛には発熱, 下痢などの典型的な症状が認められ, 血漿, 鼻汁, 糞便からウイルス遺伝子が検出された. 病理組織学的解析により, 回腸を主に腸管全域における病変が認められたが, ウイルス遺伝子および抗原検出の結果は, その他にも鼻甲介粘膜, 気管粘膜, 肺および第1-4 胃においてもウイルスが増殖することを示した.},
 pages = {19--25},
 title = {牛コロナウイルス国内流行株の抗原性比較解析と病原性試験},
 volume = {117},
 year = {2011},
 yomi = {カンノ, トオル and イシハラ, リョウコ and ハタマ, シンイチ and ウチダ, イクオ and ヤマギシ, マイコ and イシカワ, ヨシハル and カドタ, コウイチ}
}