{"created":"2023-05-15T13:38:18.203359+00:00","id":2210,"links":{},"metadata":{"_buckets":{"deposit":"1ba020f7-a2cd-4fba-9888-01afb23bb118"},"_deposit":{"created_by":12,"id":"2210","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"2210"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00002210","sets":["87:639:640:134:332"]},"author_link":["6240","1786","2122","7068"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-01-19","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"11","bibliographicPageStart":"1","bibliographicVolumeNumber":"113","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"We investigated immune responses to the P97 antigen of Mycoplasma hyopneumoniae in pigs vaccinated with Erysipelothrix rhusiopathiae YS-19 strain expressing a recombinant protein of the P97 antigen on the cell surface. In the pigs that were intranasally immunized three times with the YS-19 stain, the P97-specific serum antibodies were not detected throughout the experimental period. However, after the third immunization, peripheral blood mononuclear cells (PBMC) from the YS-19-immunized pigs significantly proliferated when stimulated with the P97 protein, compared to the cells from non-immunized pigs. Furthermore, when stimulated with the P97 protein, production of IFN-γ, an indicator of cell-mediated immune responses, from PBMC of the YS-19-immunized pigs was observed after the challenge. Irrespective of the immunization of pigs with the YS-19 strain, PBMC produced IL-8 with stimulation with P97 protein, and exuded polymorphonuclear leukocytes were observed in the bronchoalveolar lavage fluid from the pigs that had been intranasally inoculated with P97 protein. In an experiment in which a needle-free injector was used for intradermal immunization, PBMC from the pigs immunized with the YS-19 strain significantly proliferated with stimulation with the P97 protein after a challenge with a virulent M. hyopneumoniae strain. In pigs immunized with the YS-19 strain using the needle-free injector, the severity of pneumonic lung lesions caused by M. hyopneumoniae infection was significantly reduced.","subitem_description_type":"Abstract"},{"subitem_description":"Mycoplasma hyopneumoniae P97抗原の一部を菌体表面に発現する豚丹毒菌YS-19株免疫豚のP97抗原に対する免疫応答の解析を行った. YS-19株を3回経鼻的に免疫された豚では, P97抗原に対する血中抗体は検出されなかったが, 末梢血単核球は3回目の免疫直後からP97抗原刺激に対して増殖反応を示し, その程度は対照豚と比べて有意な差が認められた. また, M. hyopneumoniae強毒株攻撃接種後に採取した末梢血単核球はP97抗原刺激に対して細胞性免疫の重要な指標であるIFN-γを産生した. 免疫, 非免疫に拘わらず, 豚の末梢血単核球はP97抗原の刺激によりIL-8を産生し, P97抗原を鼻腔内に噴霧投与された豚では気管支肺胞洗浄液中に多量の好中球が観察された. さらに, YS-19株のNeedle-free injector（針なし注射）を用いたワクチン接種法の検討において, 皮内に1回免疫した豚の末梢血単核球は, M.hyopneumoniae強毒株攻撃接種後にP97抗原刺激による増殖能が有意に上昇した. なお, Needle-freeinjectorによるYS-19株免疫豚では肺炎病変の形成が有意に抑えられた.","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00002116","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00002116","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"下地, 善弘"},{"creatorName":"シモジ, ヨシヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIMOJI, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"大石, 英司"},{"creatorName":"オオイシ, エイジ","creatorNameLang":"ja-Kana"},{"creatorName":"OISHI, Eiji","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"芝原, 友幸"},{"creatorName":"シバハラ, トモユキ","creatorNameLang":"ja-Kana"},{"creatorName":"SHIBAHARA, Tomoyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"森, 康行"},{"creatorName":"モリ, ヤスユキ","creatorNameLang":"ja-Kana"},{"creatorName":"MORI, Yashuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-20"}],"displaytype":"detail","filename":"niah_report_No113p1-11p.pdf","filesize":[{"value":"468.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No113p1-11p.pdf","url":"https://repository.naro.go.jp/record/2210/files/niah_report_No113p1-11p.pdf"},"version_id":"2777a14c-afae-4b3c-8a5a-4ae0ea321dd6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Erysipelothrix rhusiopathiae YS-19","subitem_subject_scheme":"Other"},{"subitem_subject":"mycoplasmal pneumonia of swine","subitem_subject_scheme":"Other"},{"subitem_subject":"P97 antigen, vaccination","subitem_subject_scheme":"Other"},{"subitem_subject":"Erysipelothrix rhusiopathiae YS-19","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"mycoplasmal pneumonia of swine","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"P97 antigen, vaccination","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"豚丹毒菌 YS-19 株免疫豚のマイコプラズマ・ハイオニューモニエ P97 抗原に対する免疫応答の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"豚丹毒菌 YS-19 株免疫豚のマイコプラズマ・ハイオニューモニエ P97 抗原に対する免疫応答の解析"},{"subitem_title":"Immune responses to the Mycoplasma hyopneumoniae P97 antigen in pigs vaccinated with Erysipelothrix rhusiopathiae YS-19","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["332"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"2210","relation_version_is_last":true,"title":["豚丹毒菌 YS-19 株免疫豚のマイコプラズマ・ハイオニューモニエ P97 抗原に対する免疫応答の解析"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T23:03:12.787063+00:00"}