@article{oai:repository.naro.go.jp:00002181,
 author = {清水, 眞也 and SHIMIZU, Shinya and 豊田, 勇夫 and TOYODA, Isao and 有島, 太一 and ARISHIMA, Taichi and 後藤, 義之 and GOTO, Yoshiyuki},
 journal = {動物衛生研究所研究報告, Bulletin of the National Institute of Animal Health},
 month = {Jan},
 note = {Cross-reactions between bluetongue (BT) and epizootic haemorrhagic disease (EHD) virus serogroups have been reported. Ibaraki virus (IBAV), a member of EHD the serogroup, is endemic in Japan. The agar gel immunodiffusion test （AGID） is performed to survey for BT antibody in Japan. BT AGID-positive results are sometimes observed in areas of Japan in which IBAV is endemic, and this makes the interpretation of BT surveillance results difficult. However, the frequency of cross-reactions between BT virus (BTV) or IBAV-positive field serum samples against the IBA and BT AGID tests is not clear. We investigated the frequency of serological cross-reactions between IBAV and BTV on the AGID. And also, we evaluated the cross-reaction between IBA and BT in the competitive enzyme-linked immunosorbent assay (c-ELISA). The percentage of IBAV neutralisation-positive bovine serum samples that showed a positive result in the BT AGID test was 42.5%; 12.2% of the BT AGID-positive serum samples and 2.5% of the BT AGID-negative serum samples showed a positive result in the IBA AGID test. When the BT c-ELISA was used, these cross-reactions disappeared. These results indicate that serum samples from areas in which IBAV is epidemic often have positive results in the BTV AGID test, but negative results in the BTV virus neutralisation test (VNT). To obtain specific BTV surveillance results in the IBAV endemic areas, the AGID-positive results should be confirmed using the c-ELISA or VNT. (*:Immunopathology Section, Department of Immunology TEL +81-29-838-7833), 1994年わが国でのブル－タング（BT）発生以来, その血清検査には不活化BTウイルス抗原を用いたゲル内沈降反応（AGID）が実施されてきた. 時に同じオルビウイルスに属するイバラキ（IBA）ウイルスとの類属反応と考えられる陽性例が散見され, 現場において判断に混乱が生じることがある. しかし, どの程度の頻度でその類属反応が発生するかは明らかでない. そこで, IBAウイルス感染陽性牛血清を用い, BTウイルスに対する中和抗体が陰性でBTのAGIDが陽性を示す類属反応の頻度を検査した. その結果, その類属反応はIBAウイルス中和抗体価が1:4以上の血清で認められ, 42.5%の頻度で起こることが判明した. この類属反応はBTウイルス不活化濃縮抗原およびBTウイルスVP7を認識するモノクロ－ナル抗体を用いた競合ELISA（c-ELISA）では解消された. IBAウイルス感染の疑われる地域でのBTの血清診断には競合ELISAが推奨される.},
 pages = {33--38},
 title = {ブルータングゲル内沈降反応におけるイバラキウイルス感染牛血清の類属反応の発生頻度とブルータング特異的競合 ELISA の有用性},
 volume = {112},
 year = {2006},
 yomi = {シミズ, シンヤ and トヨダ, イサオ and アリシマ, タイチ and ゴトウ, ヨシユキ}
}