@article{oai:repository.naro.go.jp:00002170,
 author = {古屋, 典子 and FURUYA, Noriko and 足立, 嘉彦 and ADACHI, Yoshihiko and 塩谷, 浩一 and SHIOTANI, Hiroshi},
 journal = {果樹研究所研究報告, Bulletin of the National Institute of Fruit Tree Science},
 month = {Sep},
 note = {Citrus pseudo greasy spot (PGS) has been prevalent in Japan since the 1960s. Two basidiomycetous yeasts, Sporobolomyces productus and S. corallinus, were identified as the causative pathogens (Furuya et al., 2012). Species-specific PCR primers were designed to detect the 2 PGS pathogenic yeasts based on sequences in the ITS regions of ribosomal RNA genes of all 6 species belonging to the Subbrunneus lineage in the genus Sporobolomyces. We attempted to detect the 2 PGS pathogens from a diseased tree in a commercial field and from artificially inoculated plants by species-specific PCR. PCR did not detect S. productus; however, it detected S. corallinus from both symptomatic and asymptomatic leaves of the diseased tree. This result indicates that the yeast pathogen in the commercial field was S. corallinus. PCR amplification detected S. productus and S. corallinus from the leaves of the artificially inoculated plants 1.5 months after inoculation. However, the PCR results were negative for S. productus and faintly positive for S. corallinus from them 2 months after inoculation. In the inoculated plants, chlorotic spots were observed 2 months after inoculation, and tiny black spots surrounded by chlorosis characteristic of PGS appeared 3 months later. These results suggest that PCR detection must be performed at the early stage of infection by PGS pathogens.},
 pages = {13--18},
 title = {カンキツにせ黄斑病を引き起こす担子菌系酵母Sporobolomyces productus およびS. corallinus の種特異的プライマーを用いたPCR 法による検出},
 volume = {16},
 year = {2013},
 yomi = {フルヤ, ノリコ and アダチ, ヨシヒコ and シオタニ, ヒロシ}
}