{"created":"2026-01-08T04:11:53.275183+00:00","id":2001663,"links":{},"metadata":{"_buckets":{"deposit":"c656d21a-289e-4531-b3bb-422f152eb6cf"},"_deposit":{"created_by":121,"id":"2001663","owner":"121","owners":[121],"pid":{"revision_id":0,"type":"depid","value":"2001663"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:02001663","sets":["2:581:69","87:608:35"]},"author_link":["1708","1068","2485"],"control_number":"2001663","item_10001_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"153","bibliographicPageStart":"145","bibliographicVolumeNumber":"59","bibliographic_titles":[{"bibliographic_title":"Applied Entomology and Zoology","bibliographic_titleLang":"en"}]}]},"item_10001_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Real-time quantitative polymerase chain reaction (qPCR) was used to estimate the population densities of the potato cyst nematodes Globodera pallida Stone and Globodera rostochiensis (Wollenweber) Skarbilovich (Tylenchida: Heteroderidae). Since it is difficult to extract nematode DNA from large amounts of soil (≥ 100 g, enough for quantification of cyst nematodes), cyst isolation is required before DNA extraction. However, when isolating cysts from the soil, various impurities are simultaneously isolated, and separating the cysts from these impurities is laborious. Although previous studies have reported methods for extracting DNA from mixtures of cysts and impurities, it is unclear whether such DNA can be used to estimate nematode densities using qPCR. To examine the effects of impurities on the accuracy of qPCR quantification, we extracted DNA from nematode eggs (G. pallida and G. rostochiensis) mixed with impurities and performed qPCR. The results suggested that the differences in the fields affected the quantification accuracy. Therefore, field-specific standard curves should be set, which are impractical for routine diagnosis. To propose a more practical method, we determined a fixed standard curve for each species and estimated the population densities in field soil samples by qPCR using the standard curves. The estimated population densities significantly correlated with those determined using conventional microscopic inspections. This study revealed that the population densities of G. pallida and G. rostochiensis can be estimated from large amounts of soil, probably only approximately, but efficiently, by qPCR using DNA extracted from mixtures of cysts and impurities.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10001_description_6":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"This version of the article has been accepted for publication, after peer review (when applicable) and is subject to Springer Nature’s AM terms of use, but is not the Version of Record and does not reflect post-acceptance improvements, or any corrections. The Version of Record is available online at:　http://dx.doi.org/10.1007/s13355-024-00863-y","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Springer Nature’s AM terms of use; \nhttps://www.springernature.com/gp/open-science/policies/accepted-manuscript-terms","subitem_description_language":"en","subitem_description_type":"Other"},{"subitem_description":"Published: 24 February 2024","subitem_description_language":"en","subitem_description_type":"Other"}]},"item_10001_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer Nature","subitem_publisher_language":"en"}]},"item_10001_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1007/s13355-024-00863-y","subitem_relation_type_select":"DOI"}}]},"item_10001_relation_17":{"attribute_name":"関連サイト","attribute_value_mlt":[{"subitem_relation_name":[{"subitem_relation_name_language":"en","subitem_relation_name_text":"Publisher's version"}],"subitem_relation_type":"hasVersion","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s13355-024-00863-y","subitem_relation_type_select":"URI"}}]},"item_10001_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Authors (Author's final draft manuscript)","subitem_rights_language":"en"}]},"item_10001_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-605X","subitem_source_identifier_type":"EISSN"}]},"item_10001_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"SAKATA, Itaru","creatorNameLang":"en"},{"creatorName":"坂田, 至","creatorNameLang":"ja"},{"creatorName":"サカタ, イタル","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"SAKATA","familyNameLang":"en"},{"familyName":"坂田","familyNameLang":"ja"},{"familyName":"サカタ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Itaru","givenNameLang":"en"},{"givenName":"至","givenNameLang":"ja"},{"givenName":"イタル","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1708","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"80807404","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=80807404"},{"nameIdentifier":"sktai","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/sktai"}]},{"creatorNames":[{"creatorName":"ITO, Kenji","creatorNameLang":"en"},{"creatorName":"伊藤, 賢治","creatorNameLang":"ja"},{"creatorName":"イトウ, ケンジ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"ITO","familyNameLang":"en"},{"familyName":"伊藤","familyNameLang":"ja"},{"familyName":"イトウ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Kenji","givenNameLang":"en"},{"givenName":"賢治","givenNameLang":"ja"},{"givenName":"ケンジ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"1068","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50370515","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50370515"},{"nameIdentifier":"read0004190","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0004190"}]},{"creatorNames":[{"creatorName":"KUSHIDA, Atsuhiko","creatorNameLang":"en"},{"creatorName":"串田, 篤彦","creatorNameLang":"ja"},{"creatorName":"クシダ, アツヒコ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"KUSHIDA","familyNameLang":"en"},{"familyName":"串田","familyNameLang":"ja"},{"familyName":"クシダ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Atsuhiko","givenNameLang":"en"},{"givenName":"篤彦","givenNameLang":"ja"},{"givenName":"アツヒコ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"2485","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"40355459","nameIdentifierScheme":"e-Rad_Researcher","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=40355459"},{"nameIdentifier":"kushi36","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/kushi36"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2026-01-10"}],"displaytype":"detail","filename":"postprint_10.1007s13355-024-00863-y.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","license_note":"Springer Nature’s AM terms of use; \nhttps://www.springernature.com/gp/open-science/policies/accepted-manuscript-terms","licensetype":"license_note","mimetype":"application/pdf","url":{"url":"https://repository.naro.go.jp/record/2001663/files/postprint_10.1007s13355-024-00863-y.pdf"},"version_id":"d4d54592-df0b-45ae-b530-f408414de0df"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA extraction, Globodera spp., Impurity, Population density, Potato cyst nematodes, qPCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Efficient quantification of Globodera pallida and G. rostochiensis (Tylenchida: Heteroderidae) in large amounts of soil using probe‑based real‑time PCR","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Efficient quantification of Globodera pallida and G. rostochiensis (Tylenchida: Heteroderidae) in large amounts of soil using probe‑based real‑time PCR","subitem_title_language":"en"}]},"item_type_id":"10001","owner":"121","path":["35","69"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2026-01-10"},"publish_date":"2026-01-10","publish_status":"0","recid":"2001663","relation_version_is_last":true,"title":["Efficient quantification of Globodera pallida and G. rostochiensis (Tylenchida: Heteroderidae) in large amounts of soil using probe‑based real‑time PCR"],"weko_creator_id":"121","weko_shared_id":-1},"updated":"2026-01-08T09:04:21.245795+00:00"}