{"created":"2023-05-15T13:38:09.381222+00:00","id":1995,"links":{},"metadata":{"_buckets":{"deposit":"b2359bf7-7a39-46f5-991a-b160cf741e65"},"_deposit":{"created_by":12,"id":"1995","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1995"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001995","sets":["87:623:622:129:250"]},"author_link":["688"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2002-03-29","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"78","bibliographicPageStart":"33","bibliographicVolumeNumber":"40","bibliographic_titles":[{"bibliographic_title":"九州沖縄農業研究センター報告"},{"bibliographic_title":"Bulletin of the NARO Kyushu Okinawa Agricultural Research Center","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The application of fresh wheat or barley straw often inhibits the growth of paddy rice plants (Oryza sativa L.) in the early stages. This inhibition is generally attributed to a deficiency of available N in soils with straw added, due to considerable N assimilation by the soil microbes. Some recent reports suggested a nutrient uptake inhibition in rice plants that was not caused by or related to nutrient deficiency in the soil due to assimilation with soil microbes. Thus, the mechanism of rice growth inhibition as a result of wheat straw application has yet to be clarified beyond dispute. Studies on the formation of inhibitors of rice plant growth and N behavior in flooded soils with wheat straw added were carried out as described below to clarify the mechanism. 1. Identification of aromatic acids as inhibitors of root elongation and N uptake by rice plants cultivated in soils with wheat straw added Rice plants were cultivated with and without the application of wheat straw. The growth and N uptake of rice plants were reduced in the plots with wheat straw compared to the plots without straw. However, a larger amount of KCl-extractable N was present in the soil of the plots with straw application after 46 days of flooding, along with severe growth inhibition. In another experiment, plants and soils were sampled after 24 hours of ^15NH_4^+ application to the soil. Rice plants from soil with straw had accumulated less ^15N seventeen days after straw application, and the soil at that time contained more NH_4^+ than plants from soil without straw application. It was obvious that the decrease in N uptake by rice plants after wheat straw addition was reduced from N uptake inhibition and not from N deficiency due to assimilation by soil microbes. 2. Evaluation of the inhibitory effects of aromatic acids on rice plants' physiology We tested the inhibitory effect of fractions and individual substances obtained from soil solutions on rice root elongation. Solutions collected from flooded soil incubated with wheat straw inhibited rice root elongation, particularly the fraction that could be extracted with diethylether under acidic conditions. The acidic fraction contained aliphatic acids and aromatic acids, such as benzoic acid (BA), phenylacetic acid (PAA), 2-phenylpropionic acid (2PPA), 3-phenylpropionic acid (3PPA), 3-phenylbutyric acid (3PBA), 4-phenylbutyric acid (4PBA), 2-hydroxybenzoic acid, and t-cinnamic acid. The results of a bioassay with aromatic acids indicated strong inhibition of rice seedling growth, particularly for 2PPA and 3PPA. Further examinations were performed using ^15N-tracer in hydroponics to determine whether aromatic acids inhibit N uptake and transport in rice plants. All aromatic acids tested inhibited ^15N uptake by rice plants at concentrations of 1 (2PPA) and 100μM (3PPA and BA). Inhibitions of N transport from roots to tops by 3PPA and BA was observed at concentrations of 10 and 1000 μM, respectively. The concentrations of aromatic acids in soil solutions were determined by GC/MS. The highest concentrations in samples from rice fields (1988-1990) were 59μM (BA), 1μM (PAA), 11μM (2PPA), and 12μM (3PPA). 3. Effect of soil properties and incubation conditions on the accumulation of aromatic acids in a soil solution The effects of soil conditions and the quality of organic matter on the accumulation of aromatic acids were examined by incubating soils with wheat straw. Aromatic acids were found to be metabolite products of microbial fermentation in reduced soils. A lower (20℃) soil temperature stimulated the accumulation of aromatic acids more than a higher (40℃) temperature. Accumulations were larger in flooded than in unflooded soil. The accumulation of aromatic acids was smaller when straw was applied to soil that had already undergone five previous straw applications than when straw was added to soil without previous straw applications. 4. Conclusion The growth of rice plants under various conditions in fields or pots correlated with the aromatic acids concentration results of incubation experiments. These inhibitory effects of aromatic acids on rice plants and the patterns of their accumulation provide an important explanation for the N uptake inhibition that occurred in paddy soils with fresh wheat straw added. It was indicated that some physical properties such as the gaseous phase ratio and water permeability, which can be changed by soil management methods such as puddling or irrigation techniques, also effect inhibition of rice plant growth.","subitem_description_type":"Abstract"},{"subitem_description":"稲麦二毛作田における麦わらの施用はしばしば水稲の生育抑制に繋がる。これは, 土壌中のアンモニア態窒素が土壌微生物により有機化されることにより生じる, 水稲に吸収可能な窒素の不足 (窒素飢餓) が原因とされてきた。本研究ではこの説の問題点を指摘し, 生育阻害物質の観点から麦わら施用に伴う水稲の生育抑制機構を解析した。麦わらを施用した湛水土壌の土壌溶液から得た水稲根の伸長阻害画分を検索し, 芳香族カルボン酸類 (安息香酸, フェニル酢酸, 2-フェニルプロピオン酸, 3-フェニルプロピオン酸, 4-フェニル酪酸) と揮発性脂肪酸類を同定し, これらの水稲栽培圃場における濃度を測定した。土壌から検出された芳香族カルボン酸の水稲根伸長阻害活性は2-フェニルプロピオン酸 > フェニル酢酸 > 3-フェニルプロピオン酸 > 安息香酸であった。水稲の窒素吸収阻害活性は, 2-フェニルプロピオン酸が検定した芳香族カルボン酸中で最も低い濃度 (1μM) で示された。圃場において種子根伸長と窒素吸収の両者を阻害する濃度の2-フェニルプロピオン酸が検出された。土壌中アンモニア態窒素の減少が生じる期間は, 湛水直後の数日に限られ, 水稲の吸収抑制の比率と比較するとその割合は小さかった。麦わら施用に伴う水稲の生育抑制に関与する諸要因の中で, 芳香族カルボン酸の集積は重要な要因であると推定した。","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001909","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業技術研究機構 九州沖縄農業研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001909","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-9177","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田中, 福代"},{"creatorName":"タナカ, フクヨ","creatorNameLang":"ja-Kana"},{"creatorName":"TANAKA, Fukuyo","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-19"}],"displaytype":"detail","filename":"konarc_report_No40p33-78p.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"konarc_report_No40p33-78p.pdf","url":"https://repository.naro.go.jp/record/1995/files/konarc_report_No40p33-78p.pdf"},"version_id":"48cd7b13-0f1c-445b-aeac-67fdc8e4fffe"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"^15N トレーサー","subitem_subject_scheme":"Other"},{"subitem_subject":"水稲","subitem_subject_scheme":"Other"},{"subitem_subject":"生育抑制","subitem_subject_scheme":"Other"},{"subitem_subject":"窒素飢餓","subitem_subject_scheme":"Other"},{"subitem_subject":"窒素吸収阻害","subitem_subject_scheme":"Other"},{"subitem_subject":"芳香族カルボン酸","subitem_subject_scheme":"Other"},{"subitem_subject":"麦わら","subitem_subject_scheme":"Other"},{"subitem_subject":"Aromatic acids","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Growth inhibition","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nitrogen immobilization","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nitrogen uptake inhibition","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Rice plants","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tracer ^15N","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Wheat straw","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"水田への麦わら施用に伴う芳香族カルボン酸の生成と水稲の生育抑制機構に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"水田への麦わら施用に伴う芳香族カルボン酸の生成と水稲の生育抑制機構に関する研究"},{"subitem_title":"Formation of Aromatic Acids and Growth Inhibition of Rice (Oryza sativa L.) Plants in Flooded Soils with Wheat Straw Added","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["250"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1995","relation_version_is_last":true,"title":["水田への麦わら施用に伴う芳香族カルボン酸の生成と水稲の生育抑制機構に関する研究"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T16:11:58.399296+00:00"}