@article{oai:repository.naro.go.jp:00001960, author = {吉田, 克志 and YOSHIDA, Katsuyuki}, journal = {野菜茶業研究所研究報告, Bulletin of the National Institute of Vegetable and Tea Science}, month = {Mar}, note = {Bacterial shoot blight (BSB) caused by Pseudomonas syringae pv. theae is one of the most serious diseases of tea (Camellia sinensis L.) in Japan. BSB is epidemic from late autumn to early spring, and an outbreak can significantly reduce the yield of the first crop. However, little is known about the variations in BSB resistance among tea cultivars. This study assayed BSB resistance among tea cultivars and breeding lines in a field inoculation test. Small hedge (about 1.0m long by 1.5m wide) of 165 cultivars and breeding lines were inoculated with a suspension of P. syringae pv. theae (1 × 10^8 cfu/mL, 100mL/m^2) in late December. Infected leaves were counted in a 50-cm-square frame (4 replicates per tested plant) in late March of the next year. The inoculation tests were performed at least twice. The number of infected leaves per m^2 was calculated. Median value of the number of infected leaves in all tested plants was calculated by statistical assay at each tested years. BSB resistance of tested plants was classified by the average of the ratio to median value (ARM) of each years. It was classified into highly resistant (HR, ARM < 0.2), resistant (R, 0.2 ≤ ARM < 0.4), moderately resistant (MR, 0.4 ≤ ARM < 0.6), slightly susceptible (SS, 0.6 ≤ ARM< 1.0), susceptible (S, 1.0 ≤ ARM < 2.0), or extremely susceptible (ES, 2.0 ≤ ARM). The assay identified 17 HR or R cultivars (9 green tea and 8 black tea) and 13 HR or R breeding lines. The degree of resistance in the other 135 accessions was MR or lower. Prespraying the hedges with petroleum emulsifiable concentrate before inoculation enhanced the disease severity. These results indicate that the assay can screen for cultivars that are highly resistant to BSB for use in tea breeding.}, pages = {35--47}, title = {圃場接種試験によるチャ品種・系統の赤焼病抵抗性判定}, volume = {15}, year = {2016}, yomi = {ヨシダ, カツユキ} }