{"created":"2023-05-15T13:38:07.558986+00:00","id":1953,"links":{},"metadata":{"_buckets":{"deposit":"824c3146-e69c-441d-99d8-9ad06bf47f8f"},"_deposit":{"created_by":12,"id":"1953","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1953"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001953","sets":["87:639:640:134:328"]},"author_link":["6127","6126","1046","6124","7068","2285"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2003-03-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"42","bibliographicPageStart":"33","bibliographicVolumeNumber":"109","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Current diagnostic tests for paratuberculosis principally rest on serological assay, bacterial culture and the johnin skin test. However, diagnostic tests that are both sensitive and specific for detecting all subclinically affected animals have not yet been found. Therefore, a number of studies have been conducted in order to find rapid and accurate diagnostic methods for paratuberculosis. As a result, the following have been found; 1) PCR test with internal control DNA is accurate, sensitive and rapid for the detection of Mycobacterium avium subsp. paratuberculosis in faecal samples. 2) In the interferon gamma (IFN-γ) assay using johnin purified protein derivative (J-PPD), bovine tuberculin PPD and concanavalin A (Con A), IFN-γ responses against J-PPD were the highest in affected animals. On the contrary those of Con A were the highest in healthy animals. Interpretation of the IFN-γ assay by the higher IFN-γ responses against J-PPD than those of Con A is preferable as one of the diagnostic criteria. 3) Monoclonal antibody (711-1-1) which recognizes the lipoarabinomannan antigen of M. avium subsp. paratuberculosis did not react with M. avium subsp. avium, and showed potential usefulness in the serological tests. 4) A recombinant alkyl hydroperoxide reductase C of M. avium subsp. paratuberculosis has been prepared and successfully applied to induce IFN-γ from peripheral blood mononuclear cells of animals infected with M. avium subsp. paratuberculosis. 5) In the course of study on the role of cytokines, monocyte chemoattractant protein-1 seems to be involved in the pathogenesis of paratuberculosis.\n","subitem_description_type":"Abstract"},{"subitem_description":"ヨーネ病診断技術の改良, 開発を目的として, 1）PCRによる糞便中ヨーネ菌の検出, 2）インターフェロン・ガンマ（IFN-γ）検出によるヨーネ病の早期診断, 3）ヨーネ菌特異抗原及び表面抗原の解析と診断への応用, 4）ヨーネ菌感染における細胞性免疫応答の解析等, 様々な角度からヨーネ病の新たな診断法について検討した. PCR法として, internal control DNAを添加した反応系が, サンプル毎の糞便由来PCR反応阻害作用の影響を検定することが可能であり, 糞便中ヨーネ菌検出法として有用であった. I F N - γ検査では, 陽性対照として設けたconcanavalin A（Con A）刺激によるIFN-γの産生量が, 健康牛では最も高い値を示すのに対し, ヨーネ病感染牛ではヨーニンPPD刺激時のIFN-γ産生量が最も高い傾向が認められたことから, この点を診断上の一つの基準とすることが望ましい. ヨーネ菌の抗原解析において, 類似の鳥型結核菌とは反応しないヨーネ菌に対して特異性の高いモノクローナル抗体（Mab）が得られた. さらに, 当該Mabはヨーネ菌の表面に存在するリポアラビノマンナンを認識していた. ヨーネ菌Alkyl hydroperoxide reductases C の遺伝子組換え体を作出し, 本組換え抗原がヨーネ菌実験感染牛の血液細胞からIFN-γの産生を誘導することを確認した. ケモカインの一つであるmonocyte chemoattractant protein-1の産生とヨーネ菌感染との関連がマウス感染モデル実験において示唆された. \n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001882","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業技術研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001882","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"森, 康行"},{"creatorName":"モリ, ヤスユキ","creatorNameLang":"ja-Kana"},{"creatorName":"MORI, Yashuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"菊間, 礼子"},{"creatorName":"キクマ, レイコ","creatorNameLang":"ja-Kana"},{"creatorName":"KIKUMA, Reiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"宗田, 吉広"},{"creatorName":"ムネタ, ヨシヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"MUNETA, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"吉原, 一浩"},{"creatorName":"ヨシハラ, カズヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"YOSHIHARA, Kazuhiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"彦野, 弘一"},{"creatorName":"ヒコノ, ヒロカズ","creatorNameLang":"ja-Kana"},{"creatorName":"HIKONO, Hirokazu","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"百溪, 英一"},{"creatorName":"モモタニ, エイイチ","creatorNameLang":"ja-Kana"},{"creatorName":"MOMOTANI, Eiichi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-19"}],"displaytype":"detail","filename":"niah_report_No109p33-42p.pdf","filesize":[{"value":"120.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No109p33-42p.pdf","url":"https://repository.naro.go.jp/record/1953/files/niah_report_No109p33-42p.pdf"},"version_id":"b7041903-ebfc-4f58-aeb8-cd16593b6da0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"ヨーネ病の診断法に関する研究","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"ヨーネ病の診断法に関する研究"},{"subitem_title":"Studies on the diagnostic methods for bovine paratuberculosis","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["328"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1953","relation_version_is_last":true,"title":["ヨーネ病の診断法に関する研究"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T23:03:06.430618+00:00"}