@article{oai:repository.naro.go.jp:00001894,
 author = {加納, 健 and KANO, Takeshi and 夏秋, 知英 and NATSUAKI, Tomohide and 井坂, 正大 and ISAKA, Masahiro and SUASTIKA, Gede and SUASTIKA, Gede and 奥田, 誠一 and OKUDA, Seiichi and 家城, 洋之 and IEKI, Hiroyuki},
 journal = {果樹研究所研究報告, Bulletin of the National Institute of Fruit Tree Science},
 month = {Mar},
 note = {Several primer pairs were designed between open reading frame 6 and the 3'untranslated region for amplification of Citrus tristeza virus (CTV) complementary DNA (cDNA). Reverse transcription polymerase chain reaction (RT-PCR) followed by restriction fragment length polymorphism (RFLP) analysis of the PCR products using an appropriate combination of primer pairs and restriction enzymes was shown to be useful for distinguishing diverse CTV isolates in Japan. Three distinct experiments to investigate cross-protection against severe CTV infection were carried out using two mild isolates (Ml5A and M23A). The mild isolate Ml5A was previously reported to be a promising protective isolate. Ml5A, however, cannot be distinguished from severe isolates by means of existing monoclonal antibody techniques. Our work also demonstrates that RT-PCR-RFLP can be successfully applied to evaluation of the protective ability of mild isolates Ml5A and M23A against severe CTV isolates, since the results of RT-PCR-RFLP coincided with those of symptom observations in the cross-protection experiments., カンキツトリステザウイルス（CTV)のopen reading frame 6と3' 末端非翻訳領域の間にCTV相補DNAを増幅するためのいくつかのプライマーペアを設計した。これらのプライマーペアと制限酵素を組み合わせてRT-PCR-RFLP分析することにより、日本産の異なるCTV分離株を識別することができた。本手法がCTV弱毒株の強毒株に対する千渉効果の評価に有効であるか調べるため、弱毒株(Ml5AまたはM23A)を用いた干渉効果試験に本手法を適用した。Ml5Aは干渉効果能が高いことが報告されているが、既存のモノクローナル抗体で強毒株と識別できない分離株である。これらの試験において、RT-PCR-RFLPの結果は病徴観察結果と一致し、また、評価期間短縮の可能性が示唆された。},
 pages = {63--70},
 title = {日本産カンキツトリステザウイルス分離株のRT-PCR-RFLP分析と弱毒株の干渉効果評価への適用},
 volume = {5},
 year = {2006},
 yomi = {カノウ, タケシ and ナツアキ, トモヒデ and イサカ, マサヒロ and オクダ, セイイチOKUMU and イエキ, ヒロユキ}
}