{"created":"2023-05-15T13:38:06.059163+00:00","id":1892,"links":{},"metadata":{"_buckets":{"deposit":"631faeb2-0fa7-4eaf-a341-e64e7cf524a6"},"_deposit":{"created_by":12,"id":"1892","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1892"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001892","sets":["87:591:655:132:325"]},"author_link":["6133","6044","1576","1347","542","2048","873"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"22","bibliographicPageStart":"15","bibliographicVolumeNumber":"14","bibliographic_titles":[{"bibliographic_title":"野菜茶業研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Vegetable and Tea Science","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A post-labeling method for multiplexed genotyping analysis with a bar-coded split tag (BStag) is a useful tool to reduce labeling costs and the handling time of genotyping analyses. To apply this method to the genotyping of vegetables, we identified several BStag sequences that give no nonspecific amplified products with DNAs of various vegetable cultivars and lines. Changes to some PCR conditions, especially temperatures, also reduced nonspecific amplicons. Standard conditions using KAPA2G Fast DNA polymerase were sufficient for multiplex PCR analysis with up to four primers, but not with more than six primers. However, six to eight primers could be analyzed at once by using the KAPA2G Fast Multiplex Mix.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001838","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001838","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1346-6984","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小西, あや子"},{"creatorName":"コニシ, アヤコ","creatorNameLang":"ja-Kana"},{"creatorName":"KONISHI, Ayako","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"大山, 暁男"},{"creatorName":"オオヤマ, アキオ","creatorNameLang":"ja-Kana"},{"creatorName":"OYAMA, Akio","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"柿崎, 智博"},{"creatorName":"カキザキ, トモヒロ","creatorNameLang":"ja-Kana"},{"creatorName":"KAKIZAKI, Tomohiro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"宮武, 宏治"},{"creatorName":"ミヤタケ, コウジ","creatorNameLang":"ja-Kana"},{"creatorName":"MIYATAKE, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"山口, 博隆"},{"creatorName":"ヤマグチ, ヒロタカ","creatorNameLang":"ja-Kana"},{"creatorName":"YAMAGUCHI, Hirotaka","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"布目, 司"},{"creatorName":"ヌノメ, ツカサ","creatorNameLang":"ja-Kana"},{"creatorName":"NUNOME, Tsukasa","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"福岡, 浩之"},{"creatorName":"フクオカ, ヒロユキ","creatorNameLang":"ja-Kana"},{"creatorName":"FUKUOKA, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-19"}],"displaytype":"detail","filename":"nivts_report_No14p15-22p.pdf","filesize":[{"value":"2.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nivts_report_No14p15-22p.pdf","url":"https://repository.naro.go.jp/record/1892/files/nivts_report_No14p15-22p.pdf"},"version_id":"7f6d32cd-101a-4a93-865d-0415aa44ae96"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Bar-Coded Split Tag (BStag) を用いた DNA マーカーのポストラベル条件の検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Bar-Coded Split Tag (BStag) を用いた DNA マーカーのポストラベル条件の検討"},{"subitem_title":"Optimization of Post-Labeling Conditions for DNA Markers Using a Bar-Coded Split Tag (BStag)","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["325"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1892","relation_version_is_last":true,"title":["Bar-Coded Split Tag (BStag) を用いた DNA マーカーのポストラベル条件の検討"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:33:31.555201+00:00"}