@article{oai:repository.naro.go.jp:00001892,
 author = {小西, あや子 and KONISHI, Ayako and 大山, 暁男 and OYAMA, Akio and 柿崎, 智博 and KAKIZAKI, Tomohiro and 宮武, 宏治 and MIYATAKE, Koji and 山口, 博隆 and YAMAGUCHI, Hirotaka and 布目, 司 and NUNOME, Tsukasa and 福岡, 浩之 and FUKUOKA, Hiroyuki},
 journal = {野菜茶業研究所研究報告, Bulletin of the National Institute of Vegetable and Tea Science},
 month = {Mar},
 note = {A post-labeling method for multiplexed genotyping analysis with a bar-coded split tag (BStag) is a useful tool to reduce labeling costs and the handling time of genotyping analyses. To apply this method to the genotyping of vegetables, we identified several BStag sequences that give no nonspecific amplified products with DNAs of various vegetable cultivars and lines. Changes to some PCR conditions, especially temperatures, also reduced nonspecific amplicons. Standard conditions using KAPA2G Fast DNA polymerase were sufficient for multiplex PCR analysis with up to four primers, but not with more than six primers. However, six to eight primers could be analyzed at once by using the KAPA2G Fast Multiplex Mix.},
 pages = {15--22},
 title = {Bar-Coded Split Tag (BStag) を用いた DNA マーカーのポストラベル条件の検討},
 volume = {14},
 year = {2015},
 yomi = {コニシ, アヤコ and オオヤマ, アキオ and カキザキ, トモヒロ and ミヤタケ, コウジ and ヤマグチ, ヒロタカ and ヌノメ, ツカサ and フクオカ, ヒロユキ}
}