{"created":"2023-05-15T13:38:01.777687+00:00","id":1786,"links":{},"metadata":{"_buckets":{"deposit":"74358870-1b0d-4f87-8083-b06a7b27f505"},"_deposit":{"created_by":12,"id":"1786","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1786"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001786","sets":["87:639:640:134:333"]},"author_link":["706","5970","906","2168","5969","1280"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"14","bibliographicPageStart":"9","bibliographicVolumeNumber":"114","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Recently, atypical scrapie cases have increased mainly in genetically scrapie-resistant sheep in Europe. The characteristics of the abnormal isoform of prion protein (PrP^Sc) and its deposition pattern in the brain differ between atypical and classical scrapie sheep. During a 4-year long active surveillance of Japanese sheep and goats for transmissible spongiform encephalopathies (TSEs), 839 brains were collected; all samples were negative in western blot for the PrP^Sc of classical scrapie. In this study, we reexamined these samples by performing enzyme-linked immunosorbent assay (ELISA) in an attempt to detect atypical scrapie. No positive results were obtained in these ELISA tests. PrP genotyping revealed that most Japanese sheep are susceptible to atypical scrapie. Therefore, diagnostic and/ or surveillance tools for atypical scrapie are necessary.\n","subitem_description_type":"Abstract"},{"subitem_description":"近年, ヨーロッパ各国において非定型スクレイピー症例の増加が報告されている. 非定型スクレイピーは, 脳内に蓄積する異常プリオン蛋白質(PrP^Sc)の蛋白質分解酵素抵抗性が弱く, その分布も従来のスクレイピーとは異なっている. 本研究では, 欧州1で非定型スクレイピーの検出に応用されている固相酵素免疫測定法(ELISA) キットを用いて, 過去4年間に伝達性海綿状脳症(TSE) サーベイランスで検査された羊・山羊835検体の調査を実施した. その結果, わが国への非定型スクレイピーの侵入は確認されなかったが, 羊171検体のプリオン蛋白質のアミノ酸多型を調べたところ, その多くが非定型スクレイピーに感受性を示す遺伝子型であることが示された. このことから, 非定型スクレイピーの摘発も含めたサーベイランス手法の検討が必要と考えられた. \n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001756","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001756","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"吉田, 歩"},{"creatorName":"ヨシダ, アユム","creatorNameLang":"ja-Kana"},{"creatorName":"YOSIDA, Ayumu","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"舛甚, 賢太郎"},{"creatorName":"マスジン, ケンタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"MASUJIN, Kentaro","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"今村, 守一"},{"creatorName":"イマムラ, モリカズ","creatorNameLang":"ja-Kana"},{"creatorName":"IMAMURA, Morikazu","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"岩丸, 祥史"},{"creatorName":"イワマル, ヨシフミ","creatorNameLang":"ja-Kana"},{"creatorName":"IWAMARU, Yoshifumi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"毛利, 資郎"},{"creatorName":"モウリ, シロウ","creatorNameLang":"ja-Kana"},{"creatorName":"MOHRI, Shirou","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"横山, 隆"},{"creatorName":"ヨコヤマ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"YOKOYAMA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-19"}],"displaytype":"detail","filename":"niah_report_No114p9-14p.pdf","filesize":[{"value":"181.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No114p9-14p.pdf ","url":"https://repository.naro.go.jp/record/1786/files/niah_report_No114p9-14p.pdf"},"version_id":"29cc3d0c-0a22-4638-b071-65e47abce908"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"atypical scrapie\\","subitem_subject_scheme":"Other"},{"subitem_subject":"prion, surveillance","subitem_subject_scheme":"Other"},{"subitem_subject":"PrP genotype","subitem_subject_scheme":"Other"},{"subitem_subject":"atypical scrapie\\","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"prion, surveillance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"PrP genotype","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"わが国における非定型スクレイピーのレトロスペクティブな調査","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"わが国における非定型スクレイピーのレトロスペクティブな調査"},{"subitem_title":"Retrospective surveillance of atypical scrapie in Japan","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["333"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1786","relation_version_is_last":true,"title":["わが国における非定型スクレイピーのレトロスペクティブな調査"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:28:52.187302+00:00"}