{"created":"2023-05-15T13:38:01.300254+00:00","id":1775,"links":{},"metadata":{"_buckets":{"deposit":"c7da9a0c-bb59-4900-8f15-3c4ffcf55297"},"_deposit":{"created_by":12,"id":"1775","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1775"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001775","sets":["87:639:640:134:333"]},"author_link":["7173","5840","643","1658","5851"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2008-01-31","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"7","bibliographicPageStart":"1","bibliographicVolumeNumber":"114","bibliographic_titles":[{"bibliographic_title":"動物衛生研究所研究報告"},{"bibliographic_title":"Bulletin of the National Institute of Animal Health","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A total of 90 strains of Mycoplasma hyopneumoniae from 70 farms in 10 prefectures, collecting between 2004 and 2005, were investigated to determine in vitro susceptibilities to nine drugs that are considered as an effective antimicrobial agent for mycoplasmas. Seven strains were found resistant to 16-membered macrolide antibiotics and lincomycin. All resistant strains had point mutations either at A2058G (five strains) or A2058G and A2062C (two strains) in 23S rDNA. Strains possessed two mutations showed higher resistance to tylosin than those of one mutation. Been focused on the mutation in 23S rDNA, we developed a rapid detecting technique, using HR-1^TM and LCGreen I^TM, for the screening of macrolide resistant M hyopneumoniae. This technique is on the basis of detection of a point mutation in the limited region on 23S ribosomal RNA. It took four hours to yield a result when a strain was harvested, whereas, eight hours when a lung lesion sample was examined. ","subitem_description_type":"Abstract"},{"subitem_description":"国内各地域の10道県70農場から2004 ～ 2005年にかけて出荷された肉豚の肺炎病巣部から分離したMycoplasma hyopneumoniae 野外分離90株について薬剤感受性試験を実施した. 供試抗菌薬剤は豚呼吸器マイコプラズマ対策に有効とされる各種薬剤系9種を選択した. 薬剤耐性株は7株見いだされ, いずれもマクロライド系薬剤とリンコマイシンに交差耐性を示した. マクロライド系薬剤はマイコプラズマ感染症対策の特効薬として頻用される抗菌剤であることから, 耐性を示した7株と感受性株について分子生物学的な比較を実施したところ, 耐性株はいずれも23SリボソームRNAに1カ所(2058位A→ G,  A2058G) あるいは2カ所(A2058GおよびA2062C) のミューテーションが確認された. そこで当該領域からミューテーションを検出するDNAシーケンス法以外の, より簡便で安価な方法を検討したところPCR産物のDNA蛍光融解曲線解析法の有効性が示唆された. この方法で分離培養菌株を供試した場合, サンプルの処理から結果を得るまでに要する時間は4時間であった. また, 肺炎病巣部の乳剤を材料として実施する場合はnested PCRを実施するため, 結果を得るまでの所要時間は約8時間であった. ","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001820","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 動物衛生研究所"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001820","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-2542","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"小林, 秀樹"},{"creatorName":"コバヤシ, ヒデキ","creatorNameLang":"ja-Kana"},{"creatorName":"KOBAYASHI, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"金崎, 未香","creatorNameLang":"ja"},{"creatorName":"KANAZAKI, Mika","creatorNameLang":"en"},{"creatorName":"金﨑, 未香","creatorNameLang":"ja"},{"creatorName":"カナザキ, ミカ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"秦, 英司"},{"creatorName":"ハタ, エイジ","creatorNameLang":"ja-Kana"},{"creatorName":"HATA, Eiji","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"西森, 敬"},{"creatorName":"ニシモリ, ケイ","creatorNameLang":"ja-Kana"},{"creatorName":"NISHIMORI, Kei","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"江口, 正志"},{"creatorName":"エグチ, マサシ","creatorNameLang":"ja-Kana"},{"creatorName":"EGUCHI, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-19"}],"displaytype":"detail","filename":"niah_report_No114p1-7p.pdf","filesize":[{"value":"259.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"niah_report_No114p1-7p.pdf","url":"https://repository.naro.go.jp/record/1775/files/niah_report_No114p1-7p.pdf"},"version_id":"81c257ed-00b3-4a3b-b025-9613386fd8e4"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"high resolution melter analysis","subitem_subject_scheme":"Other"},{"subitem_subject":"macrolide-resistance","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycoplasma hyopneumoniae","subitem_subject_scheme":"Other"},{"subitem_subject":"23S rDNA.","subitem_subject_scheme":"Other"},{"subitem_subject":"high resolution melter analysis","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"macrolide-resistance","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycoplasma hyopneumoniae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"23S rDNA.","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mycoplasma hyopneumoniae の薬剤感受性試験ならびにマクロライド耐性簡易スクリーニング法の開発","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mycoplasma hyopneumoniae の薬剤感受性試験ならびにマクロライド耐性簡易スクリーニング法の開発"},{"subitem_title":"Development of a rapid screening technique for the detection of macrolide-resistant strains of Mycoplasma hyopneumoniae in swine.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["333"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1775","relation_version_is_last":true,"title":["Mycoplasma hyopneumoniae の薬剤感受性試験ならびにマクロライド耐性簡易スクリーニング法の開発"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-26T06:48:11.132504+00:00"}