{"created":"2023-05-15T13:38:00.259784+00:00","id":1748,"links":{},"metadata":{"_buckets":{"deposit":"18614bdf-c2bf-485d-9214-c58b9192768f"},"_deposit":{"created_by":12,"id":"1748","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1748"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001748","sets":["87:614:615:342:352"]},"author_link":["1105","1553","766"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-02-01","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"27","bibliographicPageStart":"13","bibliographicVolumeNumber":"10","bibliographic_titles":[{"bibliographic_title":"近畿中国四国農業研究センター研究報告"},{"bibliographic_title":"BULLETIN of THE NATIONAL AGRICULTURAL RESEARCH CENTER for WESTERN REGION","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"To establish cheap and reliable diagnostic method, in this study, we tried to apply three serological techniques, that is, ELISA, RIPA and IC-PCR assay, to the diagnosis of tomato yellow leaf curl disease. First of all, TYLCV was purified from infected tomato plants to obtain antiserum. Western blot analysis showed that the IgG purified from obtained antiserum was specific to TYLCV. DAS-ELISA detected TYLCV up to 6,400-fold diluted extract of infected tomato leaves. Meanwhile, in F(ab')_2-ELISA, the detection limit of TYLCV was 12,800-fold diluted extract. In IC-PCR assay, the dilution end point for detection of TYLCV in leaf extract was 10^7. IC-PCR assay was 1,000-fold more sensitive than F(ab')_2-ELISA. However, F(ab')_2-ELISA also could detect TYLCV from viruliferous whiteflies, and from infected plants 2 to 6 days before the onset of symptoms. By RIPA, positive red line was detected up to a 3,200-time dilution of the extracts of infected tomato leaves. RIPA also detected the virus from infected plants without symptoms at incubative stage as well as F(ab')_2-ELISA. F(ab')_2-ELISA and IC-PCR assay are considered to be very practical for diagnosing a large number of samples. On the other hand, RIPA is considered to be suitable for diagnosis by tomato growers, since it is simple and agile as a pH test paper. To hamper the spread of the disease in greenhouse, RIPA is a powerful tool for rapidly removing infected plants from greenhouses as soon as possible.\n","subitem_description_type":"Abstract"},{"subitem_description":"安価で信頼性のある診断法を確立するため, 血清学的手法, すなわち ELISA, RIPA, IC-PCR 検定をトマト黄化葉巻病の診断に適用した. まず TYLCV を純化し, 兎に免疫し抗血清を得た. 抗血清から IgG を精製しそれぞれの手法に適用した. 1. ウエスタンブロット解析の結果より, 本 IgG は TYLCV の系統にかかわらず反応する一方, TbLCJV には反応せず, 血清学的診断法に使用可能であると考えられた. 2. 本 IgG を DAS-ELISA と F(ab')_2-ELISA に適用した. TYLCV 感染トマト葉磨砕液での検出希釈限界は DAS-ELISA で6400倍, F(ab')_2-ELISA で12800倍であり, F(ab')_2-ELISA の感度が高かった. 3. F(ab')_2-ELISA はタバココナジラミの保毒検定が可能であった. また, トマト新葉部での発症2～6日前に診断が可能であった. これらの結果より F(ab')_2-ELISA は, 実用性があると判断された. 4. また, 本 IgG を IC-PCR 検定に適用した. TYLCV 感染トマト葉磨砕液での検出希釈限界は10^7倍であった. 通常の PCR 検定時に行われる DNA 精製のための煩雑な作業, そして有機溶媒や遠心器等高額機材の使用が不要なため省力的であり, 多試料検定にも有効であると考えられた. 5. さらに本 IgG を RIPA に適用した. TYLCV 感染トマト葉磨砕液での検出希釈限界は3200倍であった. 検出感度は F(ab')_2-ELISA の1/4と劣るものの, F(ab')_2-ELISA と同等の発症前診断が可能であったことから実用的な診断には有効と考えられた. 現場圃場での検定のため, ハンマーを用いた簡易な検体磨砕法を考案した. 以上の結果より, 多試料および保毒虫の検定には F(ab')_2-ELISA や IC-PCR 検定が, 圃場現場での迅速な診断にはRIPAがそれぞれ適していた.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001702","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 近畿中国四国農業研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001702","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1347-1244","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大崎, 秀樹"},{"creatorName":"オオサキ, ヒデキ","creatorNameLang":"ja-Kana"},{"creatorName":"OSAKI, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"野見山, 孝司"},{"creatorName":"ノミヤマ, コウジ","creatorNameLang":"ja-Kana"},{"creatorName":"NOMIYAMA, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"石川, 浩一"},{"creatorName":"イシカワ, コウイチ","creatorNameLang":"ja-Kana"},{"creatorName":"ISHIKAWA, Koichi","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-18"}],"displaytype":"detail","filename":"nwarc_report_No10p13-27p.pdf","filesize":[{"value":"541.7 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"nwarc_report_No10p13-27p.pdf ","url":"https://repository.naro.go.jp/record/1748/files/nwarc_report_No10p13-27p.pdf"},"version_id":"4c0d0c1d-c74c-4414-ad38-57d1ee4a4443"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DAS-ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"F (ab')_2-ELISA","subitem_subject_scheme":"Other"},{"subitem_subject":"IC-PCR","subitem_subject_scheme":"Other"},{"subitem_subject":"RIPA","subitem_subject_scheme":"Other"},{"subitem_subject":"Tomato yellow leaf curl virus","subitem_subject_scheme":"Other"},{"subitem_subject":"DAS-ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"F (ab')_2-ELISA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"IC-PCR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"RIPA","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Tomato yellow leaf curl virus","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"血清学的手法のトマト黄化葉巻病の診断への適用","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"血清学的手法のトマト黄化葉巻病の診断への適用"},{"subitem_title":"Application of Serological Techniques for the Diagnosis of Tomato Yellow Leaf Curl Disease","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["352"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1748","relation_version_is_last":true,"title":["血清学的手法のトマト黄化葉巻病の診断への適用"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:48:08.857957+00:00"}