@article{oai:repository.naro.go.jp:00001662,
 author = {宮川, 久義 and MIYAGAWA, Hisayoshi and 大野, 裕和 and OHNO, Hirokazu},
 journal = {近畿中国四国農業研究センター研究報告, BULLETIN of THE NATIONAL AGRICULTURAL RESEARCH CENTER for WESTERN REGION},
 month = {Mar},
 note = {Licorice has been widely used as a pharmaceutical, cosmetic and food additive. It is known that the constituents of licorice have both antioxidant and antimicrobial activity. This study is intended to examine the control effects on some fungal diseases of vegetables such as cucumber, tomato, and sweet pepper through application of a novel purified licorice extract and to elucidate its control mechanism. The tested extract in this paper was obtained using the final extraction process of the glycyrrhizic acid from the licorice root. This extract, named fravolicorice (TM), is a yellowish powder that exhibited antimicrobial activities in preliminary tests. 1. The fravolicorice suppressed hyphal elongation of 12 kinds of plant pathogenic fungi tested from ca. 40 to ca. 80% at the concentration of 1,000 μg/ml. This suppressive effect exceeded the conventional licorice extract. 2. The fravolicorice suppressed the number of lesions, such as frogeye leaf spot of sweet pepper, anthracnose and corynespora leaf spot of cucumber, target leaf spot of tomato and other diseases when these pathogens were co-inoculated with the fravolicorice (0.1% or 1%) or when pathogens were inoculated after application of the fravolicorice. 3. When the pathogen of corynespora leaf spot was inoculated on cucumber after the fravolicorice was applied only on the lower leaves of cucumber plants (from the first to the third or the fourth leaves from the bottom), the number of lesions that appeared on the upper leaves was fewer than on control plants. 4. Concentrations of isoliquiritigenin, liquiritigenin, their aglycon, and glycyrrhizic acid were several times higher in the fravolicorice than in the conventional licorice extract. The fravolicorice was fractionated using column chromatography (stepwise elution with EtOH) into five fractions. Combined constituents of 70% and 99%EtOH-soluble fractions showed a high disease suppressive effect on corynespora leaf spot of cucumber when co-inoculated with the pathogen.},
 pages = {55--69},
 title = {甘草抽出物を用いた野菜類茎葉病害の防除に関する研究},
 volume = {6},
 year = {2007},
 yomi = {ミヤガワ, ヒサヨシ and オオノ, ヒロカズ}
}