{"created":"2023-05-15T13:37:56.260936+00:00","id":1653,"links":{},"metadata":{"_buckets":{"deposit":"ad276ae0-d866-4e66-9eb3-5b7503f90c2a"},"_deposit":{"created_by":12,"id":"1653","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1653"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001653","sets":["87:599:601:127:220"]},"author_link":["1063","862","5780","5779","2297"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-12-25","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"55","bibliographicPageStart":"39","bibliographicVolumeNumber":"26","bibliographic_titles":[{"bibliographic_title":"中央農業総合研究センター研究報告"},{"bibliographic_title":"Bulletin of the National Agricultural Research Center","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"In Niigata Prefecture, almost all of the rice cultivar 'Koshihikari' , a good eating-quality cultivar, was replaced in 2005 by the cultivars 'Koshihikari Niigata BL No.1', 'Koshihikari Niigata BL No.2', 'Koshihikari Niigata BL No.3', 'Koshihikari Niigata BL No.4', 'Koshihikari Niigata BL No.10'（after 2008）and 'Koshihikari Niigata BL No.11'（after 2011）, which are multiline cultivars composed of blast-resistant isogenic lines, so that farmers could decrease using agricultural chemicals needed to control rice blast. Because unexpected contamination from other cultivars and outcrosses out of a certain cultivar may counteract the desired reduction of agricultural chemical use, it is necessary to maintain 'pure' seed strains (uncontaminated) of 'Koshihikari BLs'. To detect such contaminations, a 'negative' DNA marker set composed of single or multiple DNA markers is very useful. We observed that with this negative marker set, no DNA fragments were amplified by PCR when template DNA from a certain target cultivar was used, but one or more DNA fragments were detected when DNA from any other contaminant cultivar was used. To develop markers for a negative marker set from STS markers, many markers must be assessed so that proper markers can be found. In contrast, developing negative sets from SNP markers is easier because by changing the 3' -end of a primer, it is possible to select the specific genotype for which the DNA fragment will be amplified.  In this study, we developed negative marker sets for multiplex PCR for 16 major Niigata rice cultivars including 'Koshihikari BLs' using 14 SNP markers and two STS markers. Of these 14 SNP markers, 10 markers of which the 3' - end of primers were changed properly were used for one or more of negative marker sets, resulting in the effective construction of these marker sets. Each negative marker set was composed of one to four markers, and the 16 negative marker sets were composed of a total of 33 SNP markers and three STS markers. By using nine negative marker sets to discriminate hybrid seeds crossing between a target and contaminant cultivars, we found that these nine negative marker sets could detect outcrosses. Using a bulk DNA preparation method for seeds or rice powder including target and contaminant cultivars, these 16 negative marker sets could discriminate contaminated samples of which the contaminant cultivars were included in at the rate of 0.4 % – 20 %. These results confirmed　that these negative marker sets will be a useful tool　to certify 'pure' seed strains by effectively detecting　unexpected contamination and outcrosses.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001610","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構 中央農業総合研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001610","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-6738","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"田淵, 宏朗"},{"creatorName":"タブチ, ヒロアキ","creatorNameLang":"ja-Kana"},{"creatorName":"TABUCHI, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"橋本, 憲明"},{"creatorName":"ハシモト, ノリアキ","creatorNameLang":"ja-Kana"},{"creatorName":"HASHIMOTO, Noriaki","creatorNameLang":"en"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"林, 敬子"},{"creatorName":"ハヤシ, ケイコ","creatorNameLang":"ja-Kana"},{"creatorName":"HAYASHI, Keiko","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"芦川, 育夫"},{"creatorName":"アシカワ, イクオ","creatorNameLang":"ja-Kana"},{"creatorName":"ASHIKAWA, Ikuo","creatorNameLang":"en"}],"nameIdentifiers":[{},{}]},{"creatorNames":[{"creatorName":"吉田, 均"},{"creatorName":"ヨシダ, ヒトシ","creatorNameLang":"ja-Kana"},{"creatorName":"YOSHIDA, Hitoshi","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-18"}],"displaytype":"detail","filename":"narc_report_No26p39-55p.pdf","filesize":[{"value":"2.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"narc_report_No26p39-55p.pdf","url":"https://repository.naro.go.jp/record/1653/files/narc_report_No26p39-55p.pdf"},"version_id":"d8b20d96-e2ad-4ad8-af51-ffb53bc4e5d3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"新潟県水稲16品種の混入・交雑検定用DNAネガマーカーセットによるバルク検定","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"新潟県水稲16品種の混入・交雑検定用DNAネガマーカーセットによるバルク検定"},{"subitem_title":"Development of Negative Marker Sets to Detect Contamination among 16 Rice Cultivars in Niigata Prefecture and Its Application with Bulk DNA Preparation Method.","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["220"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1653","relation_version_is_last":true,"title":["新潟県水稲16品種の混入・交雑検定用DNAネガマーカーセットによるバルク検定"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-05-15T15:56:17.914206+00:00"}