{"created":"2023-05-15T13:37:55.849904+00:00","id":1644,"links":{},"metadata":{"_buckets":{"deposit":"e748b4b9-47a7-4271-a537-5e864f6189cc"},"_deposit":{"created_by":12,"id":"1644","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1644"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001644","sets":["87:599:601:127:219"]},"author_link":["3","6","5"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-28","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"37","bibliographicPageStart":"29","bibliographicVolumeNumber":"25","bibliographic_titles":[{"bibliographic_title":"中央農業総合研究センター研究報告"},{"bibliographic_title":"Bulletin of the National Agricultural Research Center","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Thionins are small, basic, and cysteine-rich antimicrobial plant peptides. We produced the rice thionin Osthi1 peptide that contains three disulfide bounds, and has a molecular weight of approximately 5 kDa, as a recombinant peptide in Escherichia coli. Recombinant Osthi1 exhibited antifungal activity against the rice blast pathogen Magnaporthe oryzae, the human pathogen Candida albicans and the yeast Saccharomyces cerevisiae, although its antimicrobial activity against the rice pathogen bacteria was less pronounced than that against fungi. These results suggest that recombinant Osthi1 could act as a potent antifungal agent. To our knowledge, this is the first report of a system of rice thionin Osthi1 production in E. coli, and the subsequent evaluation of its antimicrobial activity.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001601","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"国立研究開発法人 農業・食品産業技術総合研究機構 中央農業総合研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001601","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-6738","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"SAGEHASHI, Yoshiyuki","creatorNameLang":"en"},{"creatorName":"提箸, 祥幸","creatorNameLang":"ja"},{"creatorName":"サゲハシ, ヨシユキ","creatorNameLang":"ja-Kana"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"髙久, 洋暁"},{"creatorName":"タカク, ヒロアキ","creatorNameLang":"ja-Kana"},{"creatorName":"Takaku, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{},{},{}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":""}],"affiliationNames":[{"affiliationName":""}]}],"creatorNames":[{"creatorName":"YATOU, Osamu","creatorNameLang":"en"},{"creatorName":"矢頭, 治","creatorNameLang":"ja"},{"creatorName":"ヤトウ, オサム","creatorNameLang":"ja-Kana"},{"creatorName":"YATO, Osamu","creatorNameLang":"en"}],"familyNames":[{},{},{},{}],"givenNames":[{},{},{},{}],"nameIdentifiers":[{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-18"}],"displaytype":"detail","filename":"narc_report_No25p29-37p.pdf","filesize":[{"value":"844.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"narc_report_No25p29-37p.pdf","url":"https://repository.naro.go.jp/record/1644/files/narc_report_No25p29-37p.pdf"},"version_id":"24e13428-ea00-43ac-9a2b-9fcdcf0547c0"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"大腸菌を宿主としたイネチオニン蛋白質Osthilの生産と抗菌活性の評価","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"大腸菌を宿主としたイネチオニン蛋白質Osthilの生産と抗菌活性の評価"},{"subitem_title":"Production of the recombinant rice thionin peptide Osthi1 in　Escherichia coli and assessment of its antimicrobial activity","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["219"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1644","relation_version_is_last":true,"title":["大腸菌を宿主としたイネチオニン蛋白質Osthilの生産と抗菌活性の評価"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-20T06:18:46.938379+00:00"}