{"created":"2023-05-15T13:37:53.736392+00:00","id":1605,"links":{},"metadata":{"_buckets":{"deposit":"fe01bba4-ed26-488f-baf8-7cb36b97a5e6"},"_deposit":{"created_by":12,"id":"1605","owners":[12],"pid":{"revision_id":0,"type":"depid","value":"1605"},"status":"published"},"_oai":{"id":"oai:repository.naro.go.jp:00001605","sets":["87:599:601:127:213"]},"author_link":["6","1034","5","4","3"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-10-30","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"13","bibliographicPageStart":"1","bibliographicVolumeNumber":"19","bibliographic_titles":[{"bibliographic_title":"中央農業総合研究センター研究報告"},{"bibliographic_title":"Bulletin of the National Agricultural Research Center","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A defensin AFP1（ Antifungal peptide-1） from Brassicaceae plants exhibited antifungal activity against pathogenic filamentous fungi, such as a rice pathogen Magnaporthe oryzae. Although AFP1 from seeds exhibited high antifungal activity against M. oryzae, it is hard to collect AFP1 from seeds for large scale. We conducted the heterologous production of AFP1 using the Escherichia coli expression system, which is an economical and fast procedure for producing AFP1. Because antimicrobial activity of the defensin inhibited the growth of the host E. coli, we tried to product the protein in inclusion bodies in the host. However, the resultant protein showed no antifungal activity due to the incorrect tertiary structure. The correct tertiary structure in AFP1 is kept by the four disulfide bonds inside the AFP1 molecule. In this study, arginine and glutathione as refolding enhancers could recover the tertiary structure from the inclusion bodies. Finally, using anion-exchange chromatography, we separated successfully the AFP1 protein exhibiting antifungal activity as native AFP1.\n","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.24514/00001562","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"独立行政法人 農業・食品産業技術総合研究機構 中央農業総合研究センター"}]},"item_10002_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.24514/00001562","subitem_relation_type_select":"DOI"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1881-6738","subitem_source_identifier_type":"ISSN"}]},"item_10002_version_type_20":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"SAGEHASHI, Yoshiyuki","creatorNameLang":"en"},{"creatorName":"提箸, 祥幸","creatorNameLang":"ja"},{"creatorName":"サゲハシ, ヨシユキ","creatorNameLang":"ja-Kana"}],"familyNames":[{"familyName":"SAGEHASHI","familyNameLang":"en"},{"familyName":"提箸","familyNameLang":"ja"},{"familyName":"サゲハシ","familyNameLang":"ja-Kana"}],"givenNames":[{"givenName":"Yoshiyuki","givenNameLang":"en"},{"givenName":"祥幸","givenNameLang":"ja"},{"givenName":"ヨシユキ","givenNameLang":"ja-Kana"}],"nameIdentifiers":[{"nameIdentifier":"3","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20414617","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20414617"},{"nameIdentifier":"y-sagehashi","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/y-sagehashi"}]},{"creatorNames":[{"creatorName":"栃原, 孝志"},{"creatorName":"トチハラ, タカシ","creatorNameLang":"ja-Kana"},{"creatorName":"TOCHIHARA, Takashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"4","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"9000006790061","nameIdentifierScheme":"CiNii ID","nameIdentifierURI":"http://ci.nii.ac.jp/nrid/9000006790061"},{"nameIdentifier":"read0137092","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0137092"}]},{"creatorNames":[{"creatorName":"川田, 元滋"},{"creatorName":"カワタ, モトシゲ","creatorNameLang":"ja-Kana"},{"creatorName":"KAWATA, Motoshige","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"1034","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"20355564","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=20355564"},{"nameIdentifier":"flower","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/flower"},{"nameIdentifier":"0000-0001-7719-3371","nameIdentifierScheme":"ORCID","nameIdentifierURI":"http://orcid.org/0000-0001-7719-3371"}]},{"creatorNames":[{"creatorName":"髙久, 洋暁"},{"creatorName":"タカク, ヒロアキ","creatorNameLang":"ja-Kana"},{"creatorName":"TAKAKU, Hiroaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"5","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"70350717","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=70350717"},{"nameIdentifier":"read0208121","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/read0208121"}]},{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"YATOU, Osamu","creatorNameLang":"en"},{"creatorName":"矢頭, 治","creatorNameLang":"ja"},{"creatorName":"ヤトウ, オサム","creatorNameLang":"ja-Kana"},{"creatorName":"YATO, Osamu","creatorNameLang":"en"}],"familyNames":[{"familyName":"YATOU","familyNameLang":"en"},{"familyName":"矢頭","familyNameLang":"ja"},{"familyName":"ヤトウ","familyNameLang":"ja-Kana"},{"familyName":"YATO","familyNameLang":"en"}],"givenNames":[{"givenName":"Osamu","givenNameLang":"en"},{"givenName":"治","givenNameLang":"ja"},{"givenName":"オサム","givenNameLang":"ja-Kana"},{"givenName":"Osamu","givenNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"6","nameIdentifierScheme":"WEKO"},{"nameIdentifier":"50355575","nameIdentifierScheme":"e-Rad","nameIdentifierURI":"https://kaken.nii.ac.jp/ja/search/?qm=50355575"},{"nameIdentifier":"sakurajima","nameIdentifierScheme":"researchmap","nameIdentifierURI":"http://researchmap.jp/sakurajima"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-03-16"}],"displaytype":"detail","filename":"narc_report_No19p1-13p.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"narc_report_No19p1-13p.pdf","url":"https://repository.naro.go.jp/record/1605/files/narc_report_No19p1-13p.pdf"},"version_id":"641e3cd7-e670-443a-95ca-0dea947c6c9b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"アブラナ科植物由来デイフェンシン蛋白質AFP1の大腸菌生産と抗菌活性再生方法","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"アブラナ科植物由来デイフェンシン蛋白質AFP1の大腸菌生産と抗菌活性再生方法"},{"subitem_title":"Refolding Procedure of Antifungal AFP1 Protein, a Defensin of Brassicaceae Plants, from Inclusion Bodies Expressed in Escherichia coli","subitem_title_language":"en"}]},"item_type_id":"10002","owner":"12","path":["213"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-03-22"},"publish_date":"2019-03-22","publish_status":"0","recid":"1605","relation_version_is_last":true,"title":["アブラナ科植物由来デイフェンシン蛋白質AFP1の大腸菌生産と抗菌活性再生方法"],"weko_creator_id":"12","weko_shared_id":12},"updated":"2023-07-20T06:18:47.214019+00:00"}