@article{oai:repository.naro.go.jp:00001605,
 author = {SAGEHASHI, Yoshiyuki and 提箸, 祥幸 and 栃原, 孝志 and TOCHIHARA, Takashi and 川田, 元滋 and KAWATA, Motoshige and 髙久, 洋暁 and TAKAKU, Hiroaki and YATOU, Osamu and 矢頭, 治 and YATO, Osamu},
 journal = {中央農業総合研究センター研究報告, Bulletin of the National Agricultural Research Center},
 month = {Oct},
 note = {A defensin AFP1（ Antifungal peptide-1） from Brassicaceae plants exhibited antifungal activity against pathogenic filamentous fungi, such as a rice pathogen Magnaporthe oryzae. Although AFP1 from seeds exhibited high antifungal activity against M. oryzae, it is hard to collect AFP1 from seeds for large scale. We conducted the heterologous production of AFP1 using the Escherichia coli expression system, which is an economical and fast procedure for producing AFP1. Because antimicrobial activity of the defensin inhibited the growth of the host E. coli, we tried to product the protein in inclusion bodies in the host. However, the resultant protein showed no antifungal activity due to the incorrect tertiary structure. The correct tertiary structure in AFP1 is kept by the four disulfide bonds inside the AFP1 molecule. In this study, arginine and glutathione as refolding enhancers could recover the tertiary structure from the inclusion bodies. Finally, using anion-exchange chromatography, we separated successfully the AFP1 protein exhibiting antifungal activity as native AFP1.},
 pages = {1--13},
 title = {アブラナ科植物由来デイフェンシン蛋白質AFP1の大腸菌生産と抗菌活性再生方法},
 volume = {19},
 year = {2013},
 yomi = {サゲハシ, ヨシユキ and トチハラ, タカシ and カワタ, モトシゲ and タカク, ヒロアキ and ヤトウ, オサム}
}